Papers by Andreas Teuschl
Orthopaedic Proceedings, Aug 7, 2020
Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after... more Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in case of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly underinvestigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes do not influence the respective other cell types’ migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels in the presence of supporting cells, whereas SCs see...
Nanomaterials, 2021
The additive manufacturing of low elastic modulus alloys that have a certain level of porosity fo... more The additive manufacturing of low elastic modulus alloys that have a certain level of porosity for biomedical needs is a growing area of research. Here, we show the results of manufacturing of porous and dense samples by a laser powder bed fusion (LPBF) of Ti-Nb alloy, using two distinctive fusion strategies. The nanostructured Ti-Nb alloy powders were produced by mechanical alloying and have a nanostructured state with nanosized grains up to 90 nm. The manufactured porous samples have pronounced open porosity and advanced roughness, contrary to dense samples with a relatively smooth surface profile. The structure of both types of samples after LPBF is formed by uniaxial grains having micro- and nanosized features. The inner structure of the porous samples is comprised of an open interconnected system of pores. The volume fraction of isolated porosity is 2 vol. % and the total porosity is 20 vol. %. Cell viability was assessed in vitro for 3 and 7 days using the MG63 cell line. With...
International Journal of Molecular Sciences, 2021
The repair of large bone defects remains challenging and often requires graft material due to lim... more The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrati...
Supplemental material, DS_10.1177_0363546520926471 for Zoledronic Acid Substantially Improves Bon... more Supplemental material, DS_10.1177_0363546520926471 for Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model by Jakob E. Schanda, Claudia Keibl, Patrick Heimel, Xavier Monforte, Stefan Tangl, Xaver Feichtinger, Andreas H. Teuschl, Andreas Baierl, Christian Muschitz, Heinz Redl, Christian Fialka and Rainer Mittermayr in The American Journal of Sports Medicine
Skeletal muscle tissue engineering demonstrates a promising tool for the creation of mature muscl... more Skeletal muscle tissue engineering demonstrates a promising tool for the creation of mature muscle tissue constructs in vitro and in vivo. The aim of engineering mature muscle-like constructs ranges from substituting lost tissue after traumata, cancer ablation to conduct research on in vitro muscle disease models. Therefore the interplay of three key factors is crucial, which constitute of the right biomaterial, myogenic cells moreover mechanical stimulation. In our 3D studies we were using fibrin based hydrogels with C2C12 mouse myoblasts incorporated, as fibrin constitutes a biomaterial suitable for skeletal muscle tissue engineering. The novel bioreactor system (MagneTissue) was used to apply mechanical stimuli to our ring shaped fibrin constructs. We were able to engineer skeletal muscle-like tissue constructs via parallel alignment of fibrin fibers and cells due to the applied strain causing them to differentate into myofibers. Static strain resulted in a positive effect on myo...
Plastic and Reconstructive Surgery - Global Open, 2018
Journal of Materials Science: Materials in Medicine, 2015
Recent studies have demonstrated that combining cells with meshes prior to implantation successfu... more Recent studies have demonstrated that combining cells with meshes prior to implantation successfully enhanced hernia repair. The idea is to create a biologic coating surrounding the mesh with autologous cells, before transplantation into the patient. However, due to the lack of a prompt and robust cell adhesion to the meshes, extensive in vitro cultivation is required to obtain a homogenous cell layer covering the mesh. In this context, the objective of this publication is to manufacture meshes made of silk fibres and to enhance the cytoadhesion and cytocompatibility of the biomaterial by surface immobilization of a pro-adhesive wheat germ agglutinin (lectin WGA). We first investigated the affinity between the glycoprotein WGA and cells, in solution and then after covalent immobilization of WGA on silk films. Then, we manufactured meshes made of silk fibres, tailored them with WGA grafting and finally evaluated the cytocompatibility and the inflammatory response of silk and silk-lectin meshes compared to common polypropylene mesh, using fibroblasts and peripheral blood mononuclear cells, respectively. The in vitro experiments revealed that the cytocompatibility of silk can be enhanced by surface immobilization with lectin WGA without exhibiting negative response in terms of pro-inflammatory reaction. Grafting lectin to silk meshes could bring advantages to facilitate cell-coating of meshes prior to implantation, which is an imperative prerequisite for abdominal wall tissue regeneration using cell-based therapy.
Hernia, 2015
Introduction: Endoscopic total extraperitoneal herniorrhaphy (TEP) has emerged as a recognized su... more Introduction: Endoscopic total extraperitoneal herniorrhaphy (TEP) has emerged as a recognized surgical method for adult inguinal hernia. The evolution of a novel surgical approach was known as laparoendoscopic single-site surgery (LESS) TEP repair. Aim: A clinical comparison of a novel commercial single port with a homemade single port in endoscopic total extraperitoneal (TEP) groin hernia repair. Methods: Sixty consecutive patients undergoing laparoendoscopic single-site (LESS) TEP repair were enrolled in this trial with 31 in the homemade port group and 29 in the commercial single port group. Preoperative, intraoperative, and postoperative factors were recorded. The patients were interviewed postoperatively at outpatient clinics. Results: The demographic data were comparable between the two groups. The median operative time was longer in the homemade port group than in the commercial port group (59.4 versus 51.4 minutes, respectively, p = 0.04). The homemade port group was significantly associated with more port related malfunction than the commercial port group (19% versus 0, respectively, p = 0.02). The postoperative results were comparable between the groups regarding pain scores, analgesic requirements, complications, and post-operative convalescence. Conclusions: In conclusion, this novel commercial single port is not only associated with less intra-operative malfunctions but also improved the procedural efficiency of LESS TEP groin hernia repair. Thus, a well-designed commercial port will be of significant benefit in overcoming the current perceived procedural inefficiency of single port surgery, obviating as it does the relatively time-consuming setup and practice required when utilizing a homemade port.
Acta Biomaterialia, 2015
The generation of functional biomimetic skeletal muscle constructs is still one of the fundamenta... more The generation of functional biomimetic skeletal muscle constructs is still one of the fundamental challenges in skeletal muscle tissue engineering. With the notion that structure strongly dictates functional capabilities, a myriad of cell types, scaffold materials and stimulation strategies have been combined. To further optimize muscle engineered constructs, we have developed a novel bioreactor system (MagneTissue) for rapid engineering of skeletal muscle-like constructs with the aim to resemble native muscle in terms of structure, gene expression profile and maturity. Myoblasts embedded in fibrin, a natural hydrogel that serves as extracellular matrix, are subjected to mechanical stimulation via magnetic force transmission. We identify static mechanical strain as a trigger for cellular alignment concomitant with the orientation of the scaffold into highly organized fibrin fibrils. This ultimately yields myotubes with a more mature phenotype in terms of sarcomeric patterning, diameter and length. On the molecular level, a faster progression of the myogenic gene expression program is evident as myogenic determination markers MyoD and Myogenin as well as the Ca 2+ dependent contractile structural marker TnnT1 are significantly upregulated when strain is applied. The major advantage of the MagneTissue bioreactor system is that the generated tension is not exclusively relying on the strain generated by the cells themselves in response to scaffold anchoring but its ability to subject the constructs to individually adjustable strain protocols. In future work, this will allow applying mechanical stimulation with different strain regimes in the maturation process of tissue engineered constructs and elucidating the role of mechanotransduction in myogenesis.
Journal of Biological Chemistry, 2014
Background: Signaling pathways underlying beneficial effects of extracorporeal shock wave treatme... more Background: Signaling pathways underlying beneficial effects of extracorporeal shock wave treatment (ESWT) remain to be completely elucidated. Results: ESWT enhances cell proliferation in vitro and wound healing in vivo. Conclusion: ESWT-induced ATP release and subsequent extracellular signal-regulated kinase (ERK) activation are prerequisites for enhanced cell proliferation and wound healing. Significance: Deciphering the involved signaling cascades provides the basis for ESWT as clinical wound healing treatment.
Cell and Tissue Banking, 2013
Human amniotic membrane (hAM) is a tissue containing cells with proven stem cell properties. In i... more Human amniotic membrane (hAM) is a tissue containing cells with proven stem cell properties. In its decellularized form it has been successfully applied as nerve conduit biomaterial to improve peripheral nerve regeneration in injury models. We hypothesize that viable hAM without prior cell isolation can be differentiated towards the Schwann cell lineage to generate a possible alternative to commonly applied tissue engineering materials for nerve regeneration. For in vitro Schwann cell differentiation, biopsies of hAM of 8 mm diameter were incubated with a sequential order of neuronal induction and growth factors for 21 days and characterized for cellular viability and the typical glial markers glial fibrillary acidic protein (GFAP), S100β, p75 and neurotrophic tyrosine kinase receptor (NTRK) using immunohistology. The secretion of the neurotrophic factors brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) was quantified by ELISA. The hAM maintained high viability, especially under differentiation conditions (90.2 % ± 41.6 day 14; 80.0 % ± 44.5 day 21 compared to day 0). Both, BDNF and GDNF secretion was up-regulated upon differentiation. The fresh membrane stained positive for GFAP and p75 and NTRK, which was strongly increased after culture in differentiation conditions. Especially the epithelial layer within the membrane exhibited a change in morphology upon differentiation forming a multi-layered epithelium with intense accumulations of the marker proteins. However, S100β was expressed at equal levels and equal distribution in fresh and cultured hAM conditions. Viable hAM may be a promising alternative to present formulations used for peripheral nerve regeneration.
Acta Biomaterialia, 2014
Various tissue engineering (TE) approaches are based on silk fibroin (SF) as scaffold material be... more Various tissue engineering (TE) approaches are based on silk fibroin (SF) as scaffold material because of its superior mechanical and biological properties compared to other materials. The translation of onestep TE approaches to clinical application has generally failed so far due to the requirement of a prolonged cell seeding step before implantation. Here, we propose that the plant lectin WGA (wheat germ agglutinin), covalently bound to SF, will mediate cell adhesion in a time frame acceptable to be part of a one-step surgical intervention. After the establishment of a modification protocol utilizing carbodiimide chemistry, we examined the attachment of cells, with a special focus on adipose-derived stromal cells (ASC), on WGA-SF compared to pure native SF. After a limited time frame of 20 min the attachment of ASCs to WGA-SF showed an increase of about 17-fold, as compared to pure native SF. The lectin-mediated cell adhesion further showed an enhanced resistance to trypsin (as a protease model) and to applied fluid shear stress (mechanical stability). Moreover, we could demonstrate that the adhesion of ASCs on the WGA-SF does not negatively influence proliferation or differentiation potential into the osteogenic lineage. To test for in vitro immune response, the proliferation of peripheral blood mononuclear cells in contact with the WGA-SF was determined, showing no alterations compared to plain SF. All these findings suggest that the WGA modification of SF offers important benefits for translation of SF scaffolds into clinical applications.
Cytotherapy, 2014
Adipose-derived progenitor/stem cells (ASCs) are discussed as a promising candidate for various t... more Adipose-derived progenitor/stem cells (ASCs) are discussed as a promising candidate for various tissue engineering approaches. However, its applicability for the clinic is still difficult due to intra- and inter-donor heterogeneity and limited life span in vitro, influencing differentiation capacity as a consequence to decreased multipotency. Extracorporeal shock wave treatment has been proven to be a suitable clinical tool to improve regeneration of a variety of tissues for several decades, whereas the mechanisms underlying these beneficial effects remain widely unknown. In this study we show that human and rat adipose derived stem cells respond strongly to repetitive shock wave treatment in vitro, resulting not only in maintenance and significant elevation of mesenchymal markers (CD73, CD90, CD105), but also in significantly increased differentiation capacity towards the osteogenic and adipogenic lineage as well as toward Schwann-cell like cells even after extended time in vitro, preserving multipotency of ASCs. ESWT might be a promising tool to improve ASC quality for cell therapy in various tissue engineering and regenerative medicine applications.
The natural hydrogel fibrin is considered a very suitable scaffold material for skeletal muscle e... more The natural hydrogel fibrin is considered a very suitable scaffold material for skeletal muscle engineering since its mechanical properties can be adjusted to match those of native skeletal muscle tissue. In addition, fibrin responds to uniaxial mechanical stimulation with fibril alignment along the axis of strain, thus leading to parallel cellular patterning. To exploit this feature in a biomimetic skeletal muscle engineering approach, we have developed a novel bioreactor system (“MagneTissue”) for the rapid generation of highly aligned skeletal muscle-like tissue constructs. With this system, murine myoblasts encapsulated in ring-shaped fibrin scaffolds were subjected to static mechanical strain via magnetic force transmission, leading to cellular alignment concomitant with the patterning of the scaffold material into highly organized fibrin fibrils. Within 9 days, a parallel array of myotubes with a mature phenotype in terms of sarcomeric patterning, width and length was obtained...
Nuclear Medicine and Biology
Materials Science and Engineering: C
Advances in Experimental Medicine and Biology
Uploads
Papers by Andreas Teuschl