Borys Wrobel

Adam Mickiewicz University in Poznań, Poland, Biology, Faculty Member

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Borys Wróbel currently works on methods of preservation of neural tissue, and biologically-inspired models of computation. His background is biology and computer science. Borys graduated from the University of Gdansk (Poland) in 1997, was a Fulbright Visiting Researcher in the Salk Institute for Biological Studies in San Diego, CA, and later FEBS and EMBO Fellow at the Hebrew University of Jerusalem (Israel), Marie Curie Postdoctoral Fellow at the University of Valencia, Sciex Fellow in the Insitute of Neuroinformatics at the University of Zurich and ETHZ (Switzerland), and Kosciuszko Fellow at Oregon State University (USA).

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Replication and amplification of λ plasmids in Escherichia coli during amino acid starvation and limitation

Fems Microbiology Letters, Aug 1, 1997

It was demonstrated previously that replication of plasmids derived from bacteriophage V (so-call... more It was demonstrated previously that replication of plasmids derived from bacteriophage V (so-called V plasmids) is inhibited in wild-type Escherichia coli cells starved for isoleucine and arginine whereas it proceeds under the same conditions in relA mutants. Since replication of other replicons during the stringent or relaxed response depends on the nature of the deprived amino acid, we investigated replication of V plasmids in E. coli relA and relA 3 strains starved for different amino acids. We found that replication of V plasmids is generally inhibited during the stringent, but not relaxed, response. Differences between cells starved for different amino acids, although reproducible, were not dramatic. Amino acid starvation was previously proposed as a method for amplification of V plasmid DNA in vivo. We found that during amino acid limitation V plasmids replicate more extensively in the relA mutants than during amino acid starvation. The efficiency of plasmid DNA amplification was found to be dependent on the kind of limited amino acid; in relA 3 bacteria limited for leucine we observed about 10-fold plasmid amplification. Some V plasmid replication was also found under these conditions in the relA host. The mechanism of the stringent control of V plasmid DNA replication has already been proposed. Here the possible mechanism of the regulation of V plasmid replication during amino acid limitation is presented.

Borys Wrobel

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