Modelo MICHAELIS-MENTEN

Linewever-Burk

So (g/l) Vo(g/l*min) 1/s(l/g) 1/v(l/min/g)

5 5.700 0.200 0.175
10 8.100 0.100 0.123
15 9.300 0.067 0.108
20 10.200 0.050 0.098
25 10.700 0.040 0.093

Hawes-Woolf

So (g/l) Vo(g/l*min) S/V(min)

5 3.000 1.667
10 4.300 2.326
15 4.900 3.061
20 5.300 3.774
25 5.600 4.464

Eadie- Hofstee

So (g/l) Vo(g/l*min) V/S(min-1)

5 3.0 0.600 0.700
10 4.3 0.430
0.600
15 4.9 0.327
20 5.3 0.265 0.500
25 5.6 0.224
0.400

0.300

0.200

0.100
 0.500

0.400

0.300

0.200

0.100

0.000
2.5

Parámetros Michaelis-Menten
V max 9.32 (g/L*min)
Km 6.86 (g/L)
 1/v frente 1/s
0.200
0.180 Pendiente
0.160 f(x) = 0.5127339686336 x + 0.0727541831207891
R² = 0.999806902822324 o.origen
0.140 V max(g/l*min)
0.120 Km(g/l)
0.100
0.080
0.060
0.040
0.020
0.000
0.020 0.040 0.060 0.080 0.100 0.120 0.140 0.160 0.180 0.200 0.220

S/V frente a S
5.000
4.500
f(x) = 0.140864832110993 x + 0.945296152686612
4.000 R² = 0.999751805698065 Pendiente
3.500 o.origen
3.000 V max(g/l*min)
2.500 Km(g/l)
2.000
1.500
1.000
0.500
0.000
0 5 10 15 20 25 30

V frente a V/S
0.700

0.600
Pendiente
0.500 o.origen
V max(g/l*min)
0.400
Km(g/l)
0.300

0.200

0.100
0.500

0.400

0.300

0.200

0.100

0.000
2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
0.5127
0.0728
13.74
7.04

0.1409
0.9453
7.10
6.71

-6.8171
7.1364
7.14
6.82
t (horas) glucosa (g/L) X (g/L) ∆X (g/L) ∆t (h) ∆X/∆t (g/L*h) Xmed (g/L) μ (h^-1)
0.0 180.0 1.5
34.0 172.2 2.7 1.2 34.0 0.03529411765 2.1 0.01680672
71.4 154.8 5.3 2.6 37.4 0.06951871658 4 0.01737968
102.0 132.1 8.7 3.4 30.6 0.11111111111 7 0.01587302
112.2 121.8 10.2 1.5 10.2 0.14705882353 9.45 0.01556178
129.2 102.3 13.2 3.0 17.0 0.17647058824 11.7 0.01508296
139.4 86.7 15.5 2.3 10.2 0.22549019608 14.35 0.0157136
146.2 76.0 17.1 1.6 6.8 0.23529411765 16.3 0.01443522
153.0 64.0 18.9 1.8 6.8 0.26470588235 18 0.01470588
156.4 58.2 19.8 0.9 3.4 0.26470588235 19.35 0.01367989
166.6 39.4 22.6 2.8 10.2 0.27450980392 21.2 0.01294858
176.8 22.2 25.2 2.6 10.2 0.25490196078 23.9 0.01066535
187.0 9.6 27.1 1.9 10.2 0.1862745098 26.15 0.00712331

1/μ (h)
160

140
f(x) = 1392.86222834351 x + 50.8770107097323
120 R² = 0.990914560697416

100

80 pendiente
60
ordenada
r2
40
μmáx
20 Ks
0
0 0.01 0.02 0.03 0.04 0.05 0.06 0.07
Smed (g/L) 1/S (L/g) 1/μ (h)

176.1 0.00567859 59.5

163.5 0.00611621 57.5384615
143.45 0.00697107 63
126.95 0.00787712 64.26
112.05 0.00892459 66.3
94.5 0.01058201 63.6391304
81.35 0.01229256 69.275
70 0.01428571 68
61.1 0.01636661 73.1
48.8 0.0204918 77.2285714
30.8 0.03246753 93.7615385
15.9 0.06289308 140.384211

1392.9 (g*h/L)
50.877 (h)
0.9909
0.01965525 (h^-1)
27.3777935 (g/L)
t (horas) glucosa (g/L) X (g/L) ∆X (g/L) ∆t (h) ∆X/∆t (g/L*h) Xmed (g/L) μ (h^-1)
- - -
- - - X3-X2 t3-t2 ∆X/∆t (X2+X3)/2 (∆X/∆t)/Xmed
- - - X4-X3 t4-t2 ∆X/∆t (X3+X4)/2 (∆X/∆t)/Xmed
- - - X5-X4 t5-t4 ∆X/∆t (X4+X5)/2 (∆X/∆t)/Xmed
Smed (g/L) 1/S (L/g) 1/μ (h)

(S2+S3)/2 1/Smed 1/μ

(S3+S4)/2 1/Smed 1/μ
(S5+S6)/2 1/Smed 1/μ