Papers by Vincenzo Bertolini
Revista mexicana de ciencias forestales, Apr 26, 2024
Agro productividad, Apr 16, 2024
Objective: To Identify the endophytic fungal community of the G. skinneri root in urban populatio... more Objective: To Identify the endophytic fungal community of the G. skinneri root in urban populations, showing differences in composition between velamen and cortex to better understand the fungus-root interaction in these orchids. Design/Methodology/Approach: We collected tissues from velamen and root from five specimens of G. skinneri growing on urban trees in Tapachula, Chiapas. We extracted DNA, PCR amplified the ITS marker, sequenced on the Illumina platform followed by diversity analyses and taxonomic assignment. Results: We detected 845 OTUs that were assigned to the Fungi kingdom. Velamen and cortex share 403 OTUs, 402 were found exclusively in velamen and 40 in cortex. Besides orchid mycorrhiza forming fungi, we detected other species (e.g Alternaria sp., Beauveria sp., Fusarium sp., Glomus sp. and Tricoderma sp.) that could be involved in root physiology during development and defense against pathogens and predators. Study Limitations/Implications: Metagenomic studies provide substantial amounts of data that go beyond conventional studies. However, the information generated is still limited regarding the role of each endophyte. Findings/Conclusions: Despite these limitations, our work fills a knowledge gap because we detected endophytes that were previously unknown for G. skinneri, leading to new research questions about rootendophyte relationships.
Terra Latinoamericana, Sep 29, 2023
* Letras distintas en los tratamientos indican diferencias significativas Tukey (P < 0.05). DDT= ... more * Letras distintas en los tratamientos indican diferencias significativas Tukey (P < 0.05). DDT= días después del trasplante. * Dif ferent letters in the treatments indicate significant dif ferences Tukey's (P < 0.05). DDT= days af ter transplant.
Agro productividad, Feb 20, 2024
Objective: To isolate and classify morphologically and molecularly mycorrhizal fungi associated w... more Objective: To isolate and classify morphologically and molecularly mycorrhizal fungi associated with adult plants of Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae), distributed in different phorophytes, exotic and native trees, in the City of Tapachula, Chiapas, Mexico. Design/Methodology/Approach: We sampled roots from adult plants growing in two native phorophytes, Byrsonima crassifolia (L.) KUNTH and Tabebuia rosea (BERTOL.) BERTERO EX A. DC. and two exotic phorophytes, Terminalia catappa L. and Ficus benjamina L. located in the city's road. By the isolation of mycorrhizal strains, we had diagnosed them by morpho-physiological attributes, and molecularly (Sanger sequencing of the ITS1-4 region). Results: Forty-three fungal strains of two anamorphic mycorrhizal genus Epulorhiza and Ceratorhiza were obtained: 50% of the isolates came from plants growing in the exotic tree T. catappa with undigested pelotons and the highest molecular diversity (three contigs of the genus Tulasnella). Ficus benjamina had one molecular species shared with the native B. crassifolia. Roots growing in the native T. rosea tree, even though few isolates could be purified. Study Limitations/Implications: Even if the research was exploratory, it was possible to highlight the diverse mycorrhizal partners that urban phorophytes of G. skinneri harbor, showing their potential in the ex situ conservation of this species. Findings/Conclusions: The large number of the anamorph Epulorhiza isolates obtained from all phorophytes, reinforces previous observations suggesting that candelaria is preferentially associated with species of the Family Tulasnellaceae.
Acta Botanica Mexicana, 2020
Revista Fitotecnia Mexicana
Los hongos micorrízicos arbusculares (HMA) viven en una simbiosis mutualista con la mayoría de la... more Los hongos micorrízicos arbusculares (HMA) viven en una simbiosis mutualista con la mayoría de las plantas terrestres, mediante la cual pueden incrementar la absorción de minerales y el crecimiento de las plantas. El objetivo del presente estudio fue determinar la diversidad de HMA nativos asociados con un cafetal orgánico, y evaluar el efecto de la planta hospedera y la composición del sustrato sobre la propagación del consorcio de HMA nativos. Se recolectó una muestra compuesta de suelo en la rizosfera de arbustos de café (Coffea arabica L.) y se realizó la identificación morfológica de especies, la abundancia y la diversidad de HMA. En invernadero, se realizó la propagación del consorcio micorrízico en plantas trampa de café, frijol (Phaseolus vulgaris L.), maíz (Zea mays L.) y pasto Insurgente (Brachiaria brizantha Trin. Griseb). Para cada especie de planta trampa dos tipos de sustrato, con y sin abono orgánico, fueron inoculados con 200 g de suelo que contenía 556 esporas del c...
Trends in Horticulture
The study evaluated the aseptic establishment of Monstera acuminata Koch and Monstera deliciosa L... more The study evaluated the aseptic establishment of Monstera acuminata Koch and Monstera deliciosa Liebm (Araceae) from leaves and the induction of in vitro organogenesis of M. acuminata K. from stem discs of young shoots. For this purpose, different disinfection protocols were applied to mature leaves and young shoots, from which leaf explants of approximately 1 cm2 and stem discs of approximately 1 mm thickness were extracted. The explants were established in semi-solid media with different hormone treatments during the aseptic establishment stage and induction of organogenesis. Disinfection with 3% sodium hypochlorite (NaClO) for 20 min and 50% Murashige and Skoog[1] medium with plant tissue culture preservative (PPM) favored less oxidation in leaf explants of both species. All explants of M. deliciosa in both treatments grown in PPM-added medium and at different disinfection protocols survived, showed no contamination and more than 80% retained cellular activity up to 49 days of cu...
Sont proposées dans cet article deux nouvelles espèces de Chelyorchis collectées en Amérique Cent... more Sont proposées dans cet article deux nouvelles espèces de Chelyorchis collectées en Amérique Centrale, au centre du Guatemala. Les critères qui nous ont amenés à leur conférer le statut de nouvelles espèces reposent tant sur les différences morphologiques végétatives et florales qu'elles présentent avec les espèces connues que sur les écosystèmes respectifs. En effet ceux-ci influent sur la morphologie si l'on considèrent les adaptations par mimétisme. En outre la répartition géographique potentielle des trois espèces guatémaltèques de Chelyorchis est présentée. Abstract Two new species of Turtle-orchids, Chelyorchis (Orchidaceae: Oncidiinae) from the thorny ravines of the Chixoy and Motagua Rivers, Guatemala-Two new species of Chelyorchis are presented, described and illustrated. They were collected in Neotropical river ravines, in the centre of Guatemala. The recognition of the specific status was based on morphological differences observed with the known species as well a...
Sont proposées dans cet article deux nouvelles espèces de Chelyorchis collectées en Amérique Cent... more Sont proposées dans cet article deux nouvelles espèces de Chelyorchis collectées en Amérique Centrale, au centre du Guatemala. Les critères qui nous ont amenés à leur conférer le statut de nouvelles espèces reposent tant sur les différences morphologiques végétatives et florales qu'elles présentent avec les espèces connues que sur les écosystèmes respectifs. En effet ceux-ci influent sur la morphologie si l'on considèrent les adaptations par mimétisme. En outre la répartition géographique potentielle des trois espèces guatémaltèques de Chelyorchis est présentée. Abstract Two new species of Turtle-orchids, Chelyorchis (Orchidaceae: Oncidiinae) from the thorny ravines of the Chixoy and Motagua Rivers, Guatemala-Two new species of Chelyorchis are presented, described and illustrated. They were collected in Neotropical river ravines, in the centre of Guatemala. The recognition of the specific status was based on morphological differences observed with the known species as well a...
Con más de 10 años de trabajo de campo en la región del Soconusco, se cuenta con datos muy comple... more Con más de 10 años de trabajo de campo en la región del Soconusco, se cuenta con datos muy completos de las Orchidaceae del Soconusco, incluyendo todos los hábitats y altitudes, y algunas áreas previamente no estudiadas. Actualmente, el listado de la región incluye 295 especies, de las cuales 37 especies se encuentran en alguna categoría de riesgo en la NOM-ECOL-059-2001. Además nuestra base de datos incluye 130 especies con nula representación en la base de datos del SNIB, 165 especies con poca representación (menos que 50 puntos) en la base de datos del SNIB, 3 especies nuevas a la ciencia, 2 especies de nuevo registro para el país, varias especies endémicas a la región del Soconusco y 1 especie que se consideraba extinta pero la encontramos en la región. Comparando los datos que tenemos de las colectas históricas realizadas en la región, se cuenta también con indicaciones de extinciones de especies en la región y, en conjunto con los datos geográficos del sistema de LAIGE de ECOS...
Revista de Biología Tropical, 2017
In the Mexican economy, particularly in the Soconusco region of Chiapas, the cultivation of coffe... more In the Mexican economy, particularly in the Soconusco region of Chiapas, the cultivation of coffee generates significant inputs; however, coffee plants are susceptible to pests and diseases, so it is necessary to reinforce its production through sustainable management. In this study, we searched for the native arbuscular mycorrhizal fungi (AMF) that could be used as biofertilizers in coffee cultivation. We collected 21 soil samples coming from seven coffee plantations (Coffea canephora) in the Soconusco region Chiapas, Mexico in November 2015. We isolated the spores of AMF by the wet sieving and decanting method to quantify their abundance, richness and composition of morphospecies, as well as their relationships with soil properties. A total of 20 morphospecies and five new records of AMF were obtained, and the most frequent genera were Acaulospora and Glomus. The Toluca and Victoria sites had higher morphospecies richness (17 spp. c/u) than San Agustín, November 20 and San Luis Ne...
EUROPEAN JOURNAL OF ENVIRONMENTAL SCIENCES, 2012
to determine the reproductive status of the native orchids of the biodiversity "hotspot", Biologi... more to determine the reproductive status of the native orchids of the biodiversity "hotspot", Biological corridor tacaná-Boquerón, in the region of soconusco, southeast Mexico, which are suffering the effects of habitat degradation, unsustainable exploitation and potentially, climate change, we analysed the species richness, abundance, habitat and abiotic preferences, pollinaria transport and relation to orchid populations, of male euglossine bees (Hymenoptera: apidea: euglossini) in agroecosystems and forest fragments within the region. using volatile baits we trapped 2,480 bees, consisting of 14 species, during a total of 256 hours, of which 284 individuals (11.5%) had pollinaria of 18 orchid species adhered to their bodies. three species of Eufriesia (E. caerulescens, E. mexicana, E. rugosa) and one species of euglossa (E. villosa) were recorded for the first time. We report Eulaema meriana as the pollinator of the recently rediscovered Plectrophora alata. We did not detect habitat preferences for the species of euglossini captured, and they were frequent, or even more frequent, in intensive coffee plantations, as are many of the orchid species, which can be classified as a disturbed habitat. Bees tended to be more abundant with increasing light intensity and decreasing humidity at each site. there was little indication of pollinator specificity and the position of the pollinaria of each orchid species on the bodies of the bees was also variable. We did not recover any pollinaria from various euglossinophylic, epiphytic orchid species present in the region and three bee species showed signs of population decline. However, our results indicate that many species of orchids with this pollination syndrome are receiving pollination service within an increasingly fragmented and disturbed environment, suggesting that both the orchids and the bees are adapting to the changes.
Acta Agronómica, 2017
Guarianthe skinneri (Bateman) Dressler & W.E. Higgins., is a native orchid from Mexico, considere... more Guarianthe skinneri (Bateman) Dressler & W.E. Higgins., is a native orchid from Mexico, considered as threatened species by NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and the illegal collection during its flowering season. The aim of this research was to induce in vitro callogenesis from different type of explants, using phytoregulators, in order to look for a massive production technique to contribute to its conservation. We evaluated the leaf and pseudobulb marrow explants growing in semi-solid medium MS adding BAP, 2, 4-D, Kin, the interaction of BAP/2, 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed the pseudobulb marrow explants are more suitable for in vitro introduction in comparison to leaf explants, since they perform a lower percentage of contamination (18.8% in marrow and 73.2% in leaves). Likewise, the pseudobulb marrow explants increased callus formation (10.8%) in comparison to l...
Acta Botanica Brasilica, 2014
Orchids establish symbiosis with Rhizoctonia mycorrhizal fungi, forming the characteristic peloto... more Orchids establish symbiosis with Rhizoctonia mycorrhizal fungi, forming the characteristic pelotons within the cells of the root cortex. Under natural conditions, terrestrial and epiphytic orchids have different levels of dependence upon the fungal symbiont, although various authors have mentioned that once orchid plants reach maturity the interaction becomes weaker and intermittent. Recent evidence shows that in some epiphytic orchid species mycorrhization is constant and systematic. In three species of wild orchids from southeast Mexico, we show that mycorrhization is systematically present in roots of different ages, in the wet and dry seasons. We demonstrate that the volume of the root that is colonized depends upon the quantity of rainfall and the diameter of the root, and that rainfall also determines the presence of fresh, undigested pelotons. In very thin roots, mycorrhizal colonization occupies a considerable proportion of the cortex, whereas in thicker roots the proportion of the volume of the root cortex colonized is lower.
Acta Agronómica, 2014
Quelato de hierro y agua de coco en la germinación in vitro de Rossioglossum grande (O rchidaceae)
Mycorrhiza, 2016
Orchidaceae establish symbiotic relationships with fungi in the Rhizoctonia group, resulting in i... more Orchidaceae establish symbiotic relationships with fungi in the Rhizoctonia group, resulting in interactions beneficial to both organisms or in cell destruction in one of them (pathogenicity). Previous studies have focused mostly on terrestrial species with a few, preliminary studies, on epiphytes. To further our understanding of the molecular mechanisms involved in these symbioses, we evaluated the interaction between Oncidium sphacelatum Lindl. and the mycorrhizal fungus Thanatephorus sp. strain RG26 (isolated from a different orchid species) in vitro using morphometric and proteomic analyses. Evidence from the morphometric and microscopic analysis showed that the fungus promoted linear growth and differentiation of orchid protocorms during 98 days interaction. On day 63, protocorm development was evident, so we analyzed the physiological response of both organisms at that moment. Proteome results suggest that orchid development stimulated by the fungus apparently involves cell cycle proteins, purine recycling, ribosome biogenesis, energy metabolism, and secretion that were up-regulated in the orchid; whereas in the fungus, a high expression of proteins implicated in stress response, protein-protein interaction, and saccharides and protein biosynthesis were found in the symbiotic interaction. This is the first work reporting proteins differentially expressed in the epiphytic orchid-fungus interaction and will contribute to the search for molecular markers that will facilitate the study of this symbiosis in both wild orchids and those in danger of extinction.
Many species of native Mexican orchids are scarce in nature or in danger of extinction due to the... more Many species of native Mexican orchids are scarce in nature or in danger of extinction due to the di - sappearance of their natural habitat and illegal extra...
Two new species of Turtle-orchids, Chelyorchis (Orchidaceae: Oncidiinae) from the thorny ravines ... more Two new species of Turtle-orchids, Chelyorchis (Orchidaceae: Oncidiinae) from the thorny ravines of the Chixoy and Motagua Rivers, Guatemala – Two new species of Chelyorchis are presented, described and illustrated. They were collected in Neotropical river ravines, in the centre of Guatemala. The recognition of the specific status was based on morphological differences observed with the known species as well as their particular ecosystems which causes some morphological adaptations through mimetism. Besides, we present the potential geographical distribution of the three Guatemalan Chelyorchis species. a : manuscrit reçu le 10 février 2016, accepté le 15 mars 2016 article mis en ligne sur www.richardiana.com le 17/03/2016 – pp. 215-225 © Tropicalia ISSN 1626-3596 (imp.) 2262-9017 (élect.) XVI – mars 2016 Richardiana 215 Deux espèces nouvelles de Chelyorchis – Archila et al. Resumen Descubrimiento de nuevas orquídea-tortuga, Chelyorchis (Oncidiinae: Orchidaceae) de las barrancas espi...
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Papers by Vincenzo Bertolini