NanoTemper Technologies

Protein production teams, get ready to move faster and more efficiently to deliver high-quality recombinant proteins! We’re excited to bring you Andromeda X, now with Spectral Shift technology, to help you optimize protein expression for all of your drug discovery workflows. Learn more: https://bit.ly/3whayEf

Looking for the best approach for your binding affinity studies? Join experts Thomas Welte and Jan Schnatwinkel as they dive into fluorescent-based technologies like Spectral Shift and TRIC, discuss the best practices for selecting your fluorescent label, and answer your questions live. Register for webinar: https://lnkd.in/dR-NPXG7

Part 17 of my series of 30 biophysical techniques in 30 days! 🔬 𝐒𝐩𝐞𝐜𝐭𝐫𝐚𝐥 𝐒𝐡𝐢𝐟𝐭 𝐀𝐬𝐬𝐚𝐲 𝐟𝐨𝐫 𝐌𝐞𝐚𝐬𝐮𝐫𝐢𝐧𝐠 𝐏𝐫𝐨𝐭𝐞𝐢𝐧 𝐄𝐱𝐩𝐫𝐞𝐬𝐬𝐢𝐨𝐧 𝐚𝐧𝐝 𝐒𝐭𝐚𝐛𝐢𝐥𝐢𝐭𝐲 𝐔𝐬𝐢𝐧𝐠 𝐀𝐧𝐝𝐫𝐨𝐦𝐞𝐝𝐚 𝐒𝐩𝐞𝐜𝐭𝐫𝐚𝐥 𝐒𝐡𝐢𝐟𝐭 𝐀𝐬𝐬𝐚𝐲𝐬 have become essential for assessing relative protein expression, particularly in biologics development. Using Andromeda by NanoTemper Technologies, researchers can measure expression levels and stability with high sensitivity, without needing to purify protein samples, saving time and effort. 𝐇𝐨𝐰 𝐈𝐭 𝐖𝐨𝐫𝐤𝐬: Andromeda uses a His-tagged labeling system with tris-NTA dye. Protein-dye interactions cause fluorescence changes, offering insights into expression levels. When proteins unfold, a spectral shift occurs as the dye reacts to changes in its environment. This is ideal for screening expression conditions in various systems without purification. Andromeda's versatility extends across bacterial, insect, and mammalian cells, making it especially valuable for studying difficult-to-handle proteins like membrane proteins. 𝐅𝐫𝐨𝐦 𝐌𝐲 𝐏𝐞𝐫𝐬𝐩𝐞𝐜𝐭𝐢𝐯𝐞: 𝐏𝐫𝐨𝐬: - No Purification Needed: Andromeda allows you to assess protein stability and expression levels directly from the expression system without purifying the protein. - Versatile Across Expression Systems: The technique works across bacterial, insect, and mammalian systems, making it flexible for a variety of research needs. - Great for Membrane Proteins: Andromeda is particularly useful for assessing the expression and stability of membrane proteins, which are notoriously difficult to purify and study. 𝐂𝐨𝐧𝐬: - Labeling Required: Andromeda requires the use of His-tagged proteins and a tris-NTA dye for fluorescence detection. I believe many more labelling kits will be developed that simply use the classical purification tags. - Relative Measurement Only: The technique measures relative protein expression rather than providing precise concentration data, so it’s best used for comparative analysis. Have I used this technique? ☑️ I have used this technique ☑️ I am using this technique ☑️ I am planning to use it in the future Andromeda is one of my favorite tools, offering incredible speed and insights without needing protein purification. It’s truly transformative for biologic therapeutic optimization, helping identify lead candidates while saving time and costs. What do you think about spectral shift assays in the context of the Andromeda device? #Biophysics #ProteinExpression #SpectralShift #Andromeda #LabTech #BiologicsDevelopment #ProteinEngineering #ResearchTools #ProteinQuantification #30biophys30days 🚀 I’m Nikolay – A Relentless Explorer on the Frontier of Protein Design, Lab Automation, and Therapeutics 🚀 Driven by curiosity, I’m passionate about pioneering the latest techniques and innovations in protein research and drug discovery. Image source: Nanotemper

📣 Calling all scientists in the DACH region 📣 Join Gianluca Luigi Russo from NanoTemper Technologies at the ACRO Connect Workshop in Basel next week! 📅 When: September 24 – 8:30AM - 4:15PM 📍 Where: Novartis Campus, Basel Gianluca will be discussing the latest challenges and innovative solutions in drug development, alongside experts from Yumab GmbH, University of Bern, and ACROBiosystems. Don't miss this opportunity to connect with industry & academic experts and expand your knowledge!

When choosing a method to examine binding interactions that involve intrinsically disordered proteins (IDPs), there are a few important factors to keep in mind. IDPs are sticky, prone to aggregation, and have other unique challenges that make them tough to work with. So what should you consider when selecting an affinity measurement technique for your IDP research? 1️⃣ Biophysical vs Biochemical Assays: Some IDPs like transcription factors don't have biochemical activity, so they can't be analyzed using biochemical methods. Biophysical assays are necessary to measure binding, plus they can provide insights into potential aggregation alongside binding data. 2️⃣ Direct vs Indirect Binding Assays: IDPs interact with small molecules, DNA/RNA, and other proteins, making it essential to use a method that can assess multiple types of interactions—both direct binding and displacement assays for multi-component systems. 3️⃣ In-solution vs Immobilization-based Assays: Immobilization can alter the conformational states of IDPs, leading to inaccurate results. In-solution assays enable measurements under near-native conditions, preserving the protein's natural structure and providing more reliable data, while also allowing for measurements in the presence of large aggregates. Are you working with IDPs? Learn more about how Spectral Shift technology can help: https://lnkd.in/gmvY5jaf

Are you involved in the formulation development of novel biologics? Join expert scientist Jordan William Bye on October 10th for a webinar about formulation approaches and challenges with bispecifics. Register here: https://lnkd.in/eaMhHGCC

We’re thrilled to partner with ICE Bioscience to launch the Biophysical Joint Innovation Platform in Beijing! Together, we're advancing drug discovery with cutting-edge instruments like Dianthus with Spectral Shift technology, for ultra-sensitive, high-throughput affinity screening—enhancing hit identification and speeding up drug development.

Are you a structural biologist looking to optimize your Cryo-EM grid results with challenging membrane proteins? Scientists from the Imperiali Lab at Massachusetts Institute of Technology and Cube Biotech, collaborated with us to develop a streamlined workflow for rapid screening of copolymer nanodiscs from the NativeMP Platform with Prometheus Panta to find optimal solubilization conditions for structural analysis. Learn more in our new application note: https://lnkd.in/dDuDS-jm Special thanks to Dr. Philipp T. Hanisch, Michael Ries, Camila Costa Castro, Greg Dodge & Barbara Imperiali for such a nice collaboration!

Our mission is to enable researchers to create a world where every disease is treatable, and every paper that our customers publish brings us one step closer to that goal. Thank you to the incredible scientists who trust our technologies to advance their research 🚀

1,000,000 hours more time for drug discovery A huge number. When you are 80 years old, your life has had 700,800 hours. How is it then possible to achieve ONE million more hours for drug discovery? My family suffers from cancer. I am afraid of cancer. I don’t want to be a victim of fate. I want to do something. Act. Develop a drug vs cancer? My life my be to short. Only 700k hours. Where is my leverage? How can I have a big impact? Biophysical tools for drug discovery. We now have 20,000 users of our tools. Assume every user works 50 weeks per year. Our tools are plug and play. Robust. Easy to learn and use. Our good user experience saves everyone at least 1 hour per week. 20,000 user x 50 weeks x 1 hour saving per week. This is 1,000,000 hours per year more time. 1,000,000 hours more for science. More than a lifetime. This is just a small part of the value we bring into life sciences. And even this has a huge impact. What we do her matter. Biophysical tools matter. Biotech matters. Your science matters. Be aware of it. You are doing great! #biotechnology #entrepreneurship #startup ————————— I am Philipp - founder, CEO & business angel. My bootstrapped company NanoTemper is a global leader for biophysical tools for biotech. Like my content? Click my name + follow + 🔔