Papers by Hatice Guvenmez
Applied Biochemistry and Biotechnology, Mar 31, 2016
Magnetic Ni 0.5 Zn 0.5 Fe 2 O 4 @SiO 2 nanocomposite was prepared via the rapid combustion proces... more Magnetic Ni 0.5 Zn 0.5 Fe 2 O 4 @SiO 2 nanocomposite was prepared via the rapid combustion process, and its surface was modified to obtain amino-functionalized magnetic Ni 0.5 Zn 0.5 Fe 2 O 4 @SiO 2-NH 2 nanocomposite. The modified nanocomposite was loaded on graphene oxide (GO), on which penicillin G acylase (PGA) was covalently immobilized. The structure for docking was visualized between PGA and penicillin G using the PyMol program, which revealed the configuration of the active site. Selections of immobilization conditions including immobilization concentration and time of fixation, were explored. The catalytic performance of the immobilized PGA was characterized. The immobilized and free PGA had the highest activity at pH 8.0 and 45°C. Compared with the activity of the free PGA, the activity of the immobilized PGA was affected less by pH and temperature. The immobilized PGA exhibited the high-effective activity and good stability. V max and K m of immobilized PGA were 0.8123 mol•min −1 and 0.0399 mol•L −1 , respectively. Free PGA's V max and K m were 0.6854 mol•min −1 and 0.0328 mol•L −1. Immobilized PGA remained N70% in relative activity after 9 successive cycles.
Cukurova Medical Journal, Mar 31, 2023
Öz Purpose: Infertility is described as unexplained when all of the tests of a basic infertility ... more Öz Purpose: Infertility is described as unexplained when all of the tests of a basic infertility evaluation return within normal limits and present in 15% of infertile couples. Some studies indicate that there is an association between methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) mutations and unexplained infertility in male or female grown adults. The objective of this study was to analyze the distributions of MTHFR's C677T and A1298C genotypes in couples with unexplained fertility problems (UFP) and healthy controls. Materials and Methods: Two common variants C677T and A1298C of the MTHFR gene were screened in infertile couples (n =60 for C677T polymorphism; n=62 for A1298C polymorphism) and controls from the Cukurova region of Turkey. C677T and A1298C mutations in the MTHFR gene were detected by the SNP analysis (Fragment analysis) kit of the multiplex PCR amplification/ligation products. Homocysteine levels (in serum) were determined by the human hcy ELISA kit and folate values were determined by the Beckman coulter Unicel DxI 800 chemiluminescence test kit at the Central Laboratory of Balcali Hospital in Cukurova University. Results: In this study, an association between unexplained infertility and MTHFR C677T polymorphism was not found. However, we found an association between MTHFR A1298C polymorphism and males with UFP (%7) and controls (%19). A statistically significant difference was observed between the infertile and control groups regarding i) the folate and homocysteine values of MTHFR C677T heterozygous individuals; ii) the
Journal of Applied Biological Sciences, Aug 31, 2017
A cold active lipase producing Bacillus sp. strains were isolated from sewage of oil. Bacillus sp... more A cold active lipase producing Bacillus sp. strains were isolated from sewage of oil. Bacillus sp. strain SY-7 was determined as the best lipase producing isolate. The highest enzyme production was found at 20°C and pH 8.0 on tributyrin media. Analyses of molecular mass of the partial purified lipase was carried out by SDS-PAGE which revealed a single band as 110.5 kDa. The enzyme activity and stability were determined by spectrophotometric and titrimetric methods. The enzyme was active between pH 4.0-10.0 and 5-50°C and showed optimal activity and stability at pH 8.0 and 20°C. In the presence of BaCl 2 (4mM), KCl (4mM), AgNO 3 (4mM), CuSO4 (4mM), MgCl 2 (4mM), CaCl 2 (4mM), ZnCl 2 (4mM) and NaCl (4mM), the enzyme exhibited the following activities 105%, 100%, 100%, 89%, 105%, 95%, 100% and 100%, respectively. In the presence of Tween-20(5%), Tween-80(5%), detergent-1(5%) and detergent-6(5%) the enzyme saved its original activity. SDS(5%), detergent-2(5%) and detergent-7(5%) increased the activity 10%, 5% and 5%, respectively. Detergent-3(5%), detergent-4(5%) and detergent-5(5%) reduced its activity respectively, 14%, 14% and 52%. According to these results, SY-7 lipase shows alkaline, psychrotrophilic, cold active and stable, chelator and detergent resistant properties. Owing to these properties, this lipase can be useful in detergent industry.
Journal of Basic Microbiology, Apr 26, 2016
Gram stain-negative, motile, catalase-, and oxidase- positive strain, designated DSHK107(T) was i... more Gram stain-negative, motile, catalase-, and oxidase- positive strain, designated DSHK107(T) was isolated from soil of Cukurova University campus in Adana, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain grew at 20-42 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0) and in 0-2.0% NaCl (w/v). 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Fontibacillus; strain DSHK107(T) showed highest sequence similarity to type strains Fontibacillus aquaticus GPTSA 19(T) (97.8%) and Fontibacillus panacisegetis P11-6(T) (97.0%). The major fatty acid of strain DSHK107(T) was anteiso-C15:0 (46.7). The polar lipids of strain DSHK107(T) consisted of dihosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unknown phospholipids, four unknown lipids, three unknown glycolipids, two unknown aminophospholipids, an unknown aminolipid, and an unknown aminophosphoglycolipid. The major isoprenoid quinone was MK-7. The DNA G+C content of DSHK107(T) was 42.1 mol%. DNA-DNA hybridization showed that the strain DSHK107(T) shared low DNA-DNA relatedness with F. aquaticus DSM 17643(T) , Fontibacillus solani A4STRO4(T) , and F. panacisegetis DSM 28129(T) (47, 58, and 59.3%, respectively). Thus, our results support the placement of strain DSHK107(T) within a separate and previously unrecognized species. On the basis of a taxonomic study using a polyphasic approach, strain DSHK107(T) is considered to represent a novel species of the genus Fontibacillus, for which the name Fontibacillus pullulanilyticus sp. nov. is proposed. The type strain is DSHK107(T) (=NCCB 100560(T) = DSM 100116(T) ).
BioMed Research International, 2016
The purpose of this study was to compare the in vitro efficacy of calcium hypochlorite (Ca[OCl] 2... more The purpose of this study was to compare the in vitro efficacy of calcium hypochlorite (Ca[OCl] 2) and sodium hypochlorite (NaOCl) associated with sonic (Vibringe) irrigation system in root canals which were contaminated with Enterococcus faecalis. Material and Methods. The root canals of 84 single-rooted premolars were enlarged up to a file 40, autoclaved, inoculated with Enterococcus faecalis, and incubated for 21 days. The samples were divided into 7 groups according to the irrigation protocol: G0: no treatment; G1: distilled water; G2: 2.5% NaOCl; G3: 2.5% Ca(OCl) 2 ; G4: distilled water with sonic activation; G5: 2.5% NaOCl with sonic activation; and G6: 2.5% Ca(OCl) 2 with sonic activation. Before and after decontamination procedures microbiological samples were collected and the colony-forming units were counted and the percentages of reduction were calculated. Results. Distilled water with syringe irrigation and sonic activation groups demonstrated poor antibacterial effect on Enterococcus faecalis compared to other experimental groups (< 0.05). There was no statistically significant difference between syringe and sonic irrigation systems with Ca(OCl) 2 and NaOCl. Conclusion. The antimicrobial property of Ca(OCl) 2 has been investigated and compared with that of NaOCl. Both conventional syringe irrigation and sonic irrigation were found effective at removing E. faecalis from the root canal of extracted human teeth.
Journal of Applied Biological Sciences, Sep 17, 2021
Isolation, partial purification and characterization of a protease from an alkaliphile Bacillus s... more Isolation, partial purification and characterization of a protease from an alkaliphile Bacillus sp. strain PX6 isolated from soil sample. For this purpose, the growing and the enzyme producing abilities were researched either in different temperatures or in different pH ranges, and the optimal enzyme production was obtained at pH 8.0 and 40°C. The optimum pH and temperature of the enzyme was determined at pH 8.0 and 50°C. After a pre-incubation of 60 min at 30-50°C, original activity of the enzyme was conserved 69%, and at 60-110°C original activity of the enzyme was conserved 13%. After pre-incubation of 24 h, the original activity was increased 55% at pH 6.0-9.0, retained 88% at pH 10.0 and decreased 13% at pH 11.0-12.0. While the PX6 protease was pre-treated with 2.5 mM MgCl2, BaCl2, ZnCl2, CaCl2, MnCl2, NaCl, KCl at ambient temperature for 60 min, the original activity was retained 34%, 23%, 60%, 31%, 43%, 63%, 71%, respectively, it was induced 17% with FeCl2. With a pre-treatme...
Doğal izolatlarda imipenem (Tienam) dirençliliğinin araştırıldığı bu çalışmada toplam 1279 bakter... more Doğal izolatlarda imipenem (Tienam) dirençliliğinin araştırıldığı bu çalışmada toplam 1279 bakteri ile çalışılmış ve izolatlardan 168’inin (%13.13) imipeneme dirençli oldukları belirlenmiştir. Dirençliliğin cinslere göre dağılımı ise Pseudomonas sp.,’de %22.98, Acinetobacter sp.,’de %16.6, Klebsiella sp.,’de %2.21, Escherichia coli’de (E.coli) %1.15 ve koliformlarda (tiplendirilemeyen) %2.83 şeklindedir. Kanalizasyon izolatlarında en yüksek sıklıkta dirençlilik Acinetobacter sp.,de (%100) gözlenirken Enterobacter sp.,de dirençlilik saptanamamıştır (%100 hassas). Toprak izolatlarında sadece Pseudomonas sp.,de dirençlilik (%9.55) saptanmış diğer izolatlarda ise (Acinetobacter sp., Klebsiella sp., ve Proteus mirabilis) dirençlilik saptanamamıştır. İzolatlar arasında β-laktamaz üretimi en yüksek düzeyde topraktan izole edilen Pseudomonas sp.,de (%100) saptanırken en düşük oran kanalizasyondan izole edilen E.coli’de (%4.6) bulunmuştur
Asthma Allergy Immunology, Sep 15, 2013
Objective: The determination of airborne fungal spore concentrations can be useful in the diagnos... more Objective: The determination of airborne fungal spore concentrations can be useful in the diagnosis and follow-up of allergic diseases. The first aim was to investigate the effects of meteorologic factors on the changes of fungal spore concentrations atmosphere. The second aim was to test the usage of cell protein extracts which obtained from these atmospheric fungi in skin prick tests. Materials and Methods: The fungal spores were collected by means of a Burkard seven-day recording volumetric trap. Daily concentrations of Cladosporium and Alternaria spores have been recorded. The effects of the meteorological factors like temperature and relative humidity on the variation of spore concentrations have been investigated. Additionally, total cell protein extracts were prepared from fungi; these extracts were used in skin prick tests of 35 children with fungal allergy and 20 children as control group: the results were compared with standart fungal allergens. Results: During the study period, total 1.537.307 fungal spores were counted; 1.117.763 (73%) of them were defined as Cladosporium and 419.544 (%27) were Alternaria. The number of Cladosporium and Alternaria spores reached to maximum level at may with 314.477 of Cladosporium and 159.291 of Alternaria. The increase in number of fungal spores was detected especially in the presence of temperature between 15-25°C and in humidity of 60- 70%. The fungal extracts prepared in this study did not caused any reaction when applied in skin prick tests of the control group; in the fungal allergic children, the response of hyperemia and enduration was very similar to the those of the standart fungal allergens. Conclusion: The fungal spore concentrations in Adana were above the allergic threshold levels; and could be affected by various meteorologic factors. Additionally, when compared with the standart fungal allergens, the usage of protein extracts obtained from the atmospheric fungi in skin prick tests was found success full.
Blood, Nov 15, 2013
Introduction Mutations in JAK2 have been implicated in polycythemia vera (PV), essential thromboc... more Introduction Mutations in JAK2 have been implicated in polycythemia vera (PV), essential thrombocythemia (ES), primary myelofibrosis (PMF) as wll as other myeloproliferative disorders. Aim In this study we aimed to investigate the frequency of JAK2V617F mutation on 216 patients with hematologic malignancies in childhood and 176 patients with myeloproliferative disorders in adulthood. Materials and method Patient group consist of 164 ALL and 52 AML patients in childhood and 79 PV, 51 ES, 22 chronic myeloid leukemia patients (CML) and 24 PMF patients in adulthood. These patients followed by Cukurova University, Departments of Pediatric and Adult Hematology, are included in this study. Blood samples were collected in these patients group and DNA was isolated using high pure template preparation kit (Roche) and stored -80oC. Gene mutations were studied using TMB (TıbMolBiol) LightMix Kit JAK2V617F genomic and analyzed by Light Cycler 2.0 Roche Diagnostic, GmBh, Germany in both groups. Findings JAK2V617F mutation was found 1 of 164 ALL patients (0,6%), 0 of 52 AML patients (0%) in childhood. Nevertheless, JAK2V617F mutation was also found 71 of 79 PV patients (89,8%), 22 of 51 ET patients (43,1%), 1 of 22 CML patients (4,5%) and 15 of 24 PMF patients (62,5%) in adulthood. Result As a result we found high frequency of JAK2V617F mutation in PV patients than the other myeloproliferative disorders. JAK2V617F mutation was significantly high in myeloproliferative disorders in adulthood comparing with childhood acute leukemias…
World Allergy Organization Journal, Nov 1, 2007
Little is known about the contribution of indoor molds to the symptoms of asthma and/or rhinitis ... more Little is known about the contribution of indoor molds to the symptoms of asthma and/or rhinitis in children monosensitized to molds. We aimed to investigate the effect of indoor mold spore concentrations on daily symptoms of asthma and/or rhinitis in children monosensitized to molds. Nineteen children with asthma and/or rhinitis sensitized only to molds recorded their daily symptoms and peak expiratory flow (PEF) values to the diaries, from February 2005 to January 2006. In this study period, indoor mold concentrations were measured monthly from the living rooms/bedrooms. The median indoor mold concentration was 37.5 CFU/m(3). Most commonly recovered indoor molds were Cladosporium (26.4%), Penicillium (24.7%), and Aspergillus (7%). Significant correlation was not found between indoor mold concentrations and daily rhinitis score (r = -0.021, p = 0.932), daily asthma score (r = 0.155, p = 0.554), daily morning PEF (r = -0.056, p = 0.475), and evening PEF (r = -0.057, p = 0.471). The effect of indoor molds is not evident on the symptoms of our patients with asthma and/or rhinitis monosensitized to molds.
Current Opinion in Biotechnology, Jul 1, 2013
European Biotechnology Congress -- MAY 16-18, 2013 -- Comenius Univ, Bratislava, SLOVAKIAWOS: 000... more European Biotechnology Congress -- MAY 16-18, 2013 -- Comenius Univ, Bratislava, SLOVAKIAWOS: 000323298100228…European Biotechnol Themat Network Assoc (EBTNA), Comenius Univ, Fac Nat Sc
Turkish Journal of Biology, 1996
Objective: We carried out three consecutive projects in our region over the last 15 years in orde... more Objective: We carried out three consecutive projects in our region over the last 15 years in order to assess changes in the prevalence of asthma and allergic diseases and to investigate the relationship between the allergic diseases and possible risk factors. In the last survey, we compared two different questionnaire techniques - the direct parent-reported and the doctor guided-parent reported questionnaires-in determining the risk factors for allergic diseases among school-age children. Materials and Methods: ISAAC questionnaires were given to 5150 school-age children (6-14 years) and 3470 (67.3%) of these were returned. The parents completed a simple two-page written questionnaire about asthma, allergic rhinoconjunctivitis and atopic eczema and a one-page questionnaire about the risk factors. According to the questionnaire results, we constituted an atopic group of 100 children, and 100 children with no allergic symptoms were chosen randomly as the control group. Skin prick test,...
Allergologia et Immunopathologia, 2006
Background: Approximately 150 species of Lepidoptera have been described as causing damage to hum... more Background: Approximately 150 species of Lepidoptera have been described as causing damage to human skin. One of these species is the pine processionary caterpillar, which is responsible for dermatitis, contact urticaria, ocular lesions and rarely respiratory signs and anaphylactic reactions through IgE-mediated or non-IgE-mediated mechanisms. We report a pediatric case of severe orofacial edema mimicking an allergic reaction after ingestion of a pine processionary caterpillar; urgent airway intubation was required. Case report: A 15-month-old boy was sleeping under a pine tree when his mother noted a pine caterpillar on his tongue. Because of rapidly developing facial swelling and respiratory distress, the infant was first taken to a local hospital where he received intravenous dexamethasone and pheniramine hydrogen maleate. On arrival at our emergency department, diffuse swelling and edema involving the tongue, perioral, nasal and perimandibular regions, and neck was noted, requiring urgent orotracheal intubation. There were no findings of anaphylaxis. The results of skin prick tests and specific IgE to common aero-and food allergens were negative. A skin prick test with extract of pine caterpillar was also negative. Prednisolone and pheniramine hydrogen maleate were administered for 7 days. The child gradually improved and was successfully extubated 4 days later. Conclusion: Although oral contact with a pine processionary caterpillar in the form of ingestion is rare, it may cause significant local reaction and airway compromise mimicking an allergic event. In this situation, early intubation to maintain airway patency is a life-saving measure.
Astım allerji immünoloji, 2013
Keratinases originating from microorganisms are used in many industrial fields such as the recycl... more Keratinases originating from microorganisms are used in many industrial fields such as the recycle of keratinous wastes, leather, textile, the detergent industry and medical applications. In this study, 42 Bacillus strain were isolated from Cukurova University Research and Application and Chicken Management Unit. 8 of these isolates showed proteolytic activity on skim-milk and keratinolytic activity with keratin-azure on the basal feather-meal medium. Strain H62 with the highest keratinase activity was determined as the keratinase producer and identified as Bacillus licheniformis with microscopic, biochemical (VITEK-2, 90%) and molecular analysis (16S rRNA, 99%, B. licheniformis 9945A). The highest enzyme production was carried out at 40°C for 45 hours by adding 0.1 g/l mannitol (as carbon source), 0.1 g/l ammonium nitrate (as nitrogen source) and 15 g/l feather- meal into the basal feather-meal medium. Although keratinase showed the activity at 20-90°C and pH 5.0-13.0, optimum acti...
Uploads
Papers by Hatice Guvenmez