Papers by Dildar Konukoğlu
Nephrology Dialysis Transplantation
Background and Aims The main mechanism of therapeutic action of mesenchymal stem cells (MSCs) cou... more Background and Aims The main mechanism of therapeutic action of mesenchymal stem cells (MSCs) could be resulted from their secretome content rather than their direct differentiation into damaged cells [1, 2]. Preconditioning of MSCs with different strategies like culturing in 3 dimensional (3D) culture scaffolds could improve their therapeutic potential [1]. Based on our previous study [1] in which the concentrations of total protein and specifically VEGF and IL-4 in conditioned mediums (CMs) collected from MSCs cultured in 3D culture conditions were increased compared to one of 2D, the aim of this study is comparative analysis of effects of CMs obtained from MSCs incubated under 2D and 3D culture conditions on kidney functions of rats with autoimmune type I diabetes (aT1D) induced with streptozocin (STZ). Method Isolation and characterization of the umbilical cord MSCs and the following analysis of concentrations of total protein and paracrine factors in the 2D-CMs and 3D-CMs were ...
BMC Pulmonary Medicine
Background Pulmonary embolism (PE) is a common and potentially life-threatening disorder. Our stu... more Background Pulmonary embolism (PE) is a common and potentially life-threatening disorder. Our study was aimed to investigate whether oxidative stress markers can be used as clinical markers in the evaluation of acute PE (APE) severity. Methods 47 patients with objectively documented diagnosis of APE were recorded. Of these patients, 14 had low-risk PE, 16 had moderate-risk PE, and 17 had high-risk PE. 21 healthy subjects were also enrolled in this study. Ischemia-modified albumin (IMA), prooxidants-antioxidants balance (PAB), advanced protein oxidation products (AOPPs), and ferric reducing antioxidant power (FRAP) were measured as oxidative stress parameters to evaluate the role of oxidative stress. Results In the low-risk and moderate-risk APE groups, AOPPs and PAB levels were significantly higher and FRAP levels were significantly lower than those in the control group. AOPPs and IMA levels in the patients with high-risk PE were significantly higher than those in both the low-risk ...
Stem Cell Research & Therapy
Background The therapeutic potential of mesenchymal stem cells (MSCs)-derived conditioned media (... more Background The therapeutic potential of mesenchymal stem cells (MSCs)-derived conditioned media (CM) can be increased after preconditioning with various chemical agents. The aim of this study is comparative evaluation of effects of N-CM and DFS-CM which are collected from normal (N) and deferoxamine (DFS) preconditioned umbilical cord-derived MSCs on rat diabetic nephropathy (DN) model. Methods After incubation of the MSCs in serum-free medium with/without 150 µM DFS for 48 h, the contents of N-CM and DFS-CM were analyzed by enzyme-linked immunosorbent assay. Diabetes (D) was induced by single dose of 55 mg/kg streptozotocin. Therapeutic effects of CMs were evaluated by biochemical, physical, histopathological and immunohistochemical analysis. Results The concentrations of vascular endothelial growth factor alpha, nerve growth factor and glial-derived neurotrophic factor in DFS-CM increased, while one of brain-derived neurotrophic factor decreased in comparison with N-CM. The creati...
Blood Purification, 2022
Introduction: Interleukin-6 (IL-6) is one of the most important mediators of inflammation. It is ... more Introduction: Interleukin-6 (IL-6) is one of the most important mediators of inflammation. It is also the culprit for a severe disease course in COVID-19. While COVID-19 has higher mortality in hemodialysis (HD) patients, medium cutoff (MCO) membranes were previously suggested as promising tools for better patient outcomes by purging inflammatory mediators. The aim of this study was to analyze changes in IL-6 levels of HD patients who were dialyzed via MCO membranes during their COVID-19 treatments. Methods: This is an observational study on a group of HD patients who were admitted with COVID-19 diagnosis in a university hospital and intermittently dialyzed using MCO membranes during their hospital stay. IL-6 levels of the patients were measured before and after consecutive dialysis sessions by a commercial kit. Measurements were interpreted together with the clinical data. Results: Nine patients with a total of 54 measurements were evaluated. IL-6 levels were significantly higher i...
The effects of renal replacement therapy on plasma, asymmetric dimethylarginine, nitric oxide and... more The effects of renal replacement therapy on plasma, asymmetric dimethylarginine, nitric oxide and C-reactive protein levels
Turkish Association of Pediatric Surgeons, 2018
Clinical and investigative medicine. Medecine clinique et experimentale, Jan 19, 2017
Hyperglycemia is the major risk factor for microvascular complications in type 2 diabetes mellitu... more Hyperglycemia is the major risk factor for microvascular complications in type 2 diabetes mellitus (T2DM) patients. This randomized controlled clinical trial aimed to investigate T2DM patients with microvascular complications with regard to possible relations among serum clusterin (CLU), amylin, secreted frizzled-related protein-4 (SFRP-4), glucagon-like peptide-1 (GLP-1) and dipeptidyl peptidase-4 (DPP-4) activities. Subject groups were defined as follows: T2DM without complications (n=25, F/M=9/16, age 53.9±11.1 years); T2DM+Retinopathy (n=25, F/M=13/12, age 63.8±7.1 years); T2DM+Nephropathy (n=25, F/M=13/12, age 58.7±14.4 years); T2DM+Neuropathy (n=25, F/M=15/10, age 63.2±9.6 years); and healthy control subjects (HC) (n=25). CLU, amylin, SFRP-4, DPP-4 and GLP-1 (total and active) activities were measured and compared in blood samples from type 2 diabetic patients with and without microvascular complications. Significantly lower levels of DPP-4 and GLP-1total (P.
Clinical & Investigative Medicine, 2008
Purpose: Asymmetric dimethylarginine (ADMA), nitric oxide (NOx), and C-reactive protein (CRP) are... more Purpose: Asymmetric dimethylarginine (ADMA), nitric oxide (NOx), and C-reactive protein (CRP) are important risk factors for endothelial dysfunction and mortality in the end stage renal diseases population. The aim of the study was to investigate the relationship between renal replacement therapy and endothelial dysfunction. Methods: Plasma NOx, ADMA and CRP levels were examined in randomized selected 30 patients with chronic kidney diseases (CKD), 28 patients receiving continuous ambulatory peritoneal dialysis (PD) and 30 patients receiving regular hemodialysis (HD) and age-matched 20 healthy controls. The duration of dialysis was from 4, 5 to 11, and 6 years, respectively. Results: CKD patients had higher plasma ADMA (1.26±0.53?mol/L) and CRP levels (1.02±025mg/L) and lower NOx levels (28.6±5.4?mol/L) than controls (0.45±0.20; 0.65± 0.45; 32.5±37 respectively, P < 0.001).Plasma NOx and CRP levels were higher in HD patients (32.9±5.5?mol/L, P < 0.05 and 4.59±3.18mg/L, P < ...
Minerva medica, 2013
The aim of this paper was to evaluate the effects of dialysis procedures on oxidative stress in d... more The aim of this paper was to evaluate the effects of dialysis procedures on oxidative stress in diabetic patients. The study was performed on 15 non-diabetic hemodialysis (HD) patients, 30 non-diabetic perinoteal dialysis (PD) patients, 18 diabetic HD patients (DHD), 15 diabetic PD patients (DPD), and 20 healthy controls. Plasma thiobarbituric acid reactive substances (TBARS), protein carbonyl (PCO), and oxidized LDL (oxLDL) were determined as oxidative stress markers. Plasma thiol (P-SH), erythrocyte glutathione (GSH) levels, and serum paraoxonase (PON1) activities were measured as antioxidants. HD patients have significantly higher oxLDL, TBARS and PCO levels and significantly lower P-SH levels than PD patients. DHD patients have significantly higher PCO levels and PON1 activities and significantly lower GSH levels than non-diabetic HD patients. There was no any difference in oxidative stress parameters between DPD and non-diabetic PD patients. Oxidative stress is exacerbated by H...
World Journal of Surgery, 2008
Pharmacological Research, 1999
Kathmandu University Medical Journal, 2012
Background Metabolic syndrome is a constellation of physical conditions and metabolic abnormaliti... more Background Metabolic syndrome is a constellation of physical conditions and metabolic abnormalities, commonly occurring together, that increases an individual’s risk for development of type 2 diabetes mellitus and cardiovascular diseases. Oxidative stress is associated with diabetes, hypertension and other cardiovascular diseases while the role of oxidative stress in pathogenesis of MS is not clearlydefined. Objectives The study aims to find out the prevalence of metabolic syndrome in faculty and staff members at BP Koirala Institute of Health Sciences, Dharan, Nepal and to evaluate oxidative stress levels insubjects with metabolic syndrome. Methods 118 healthy participants working at B. P. Koirala Institute of Health Sciences, Dharan, Nepal were selected at random for this cross-sectional study and blood samples were collected for the estimation of the following biochemical analytes; fasting glucose; triglycerides; total cholesterol; high density lipoprotein cholesterol; Albumin; u...
Journal of the American College of Cardiology, 2010
European Journal of Obstetrics & Gynecology and Reproductive Biology, 1999
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Ulus Travma Acil …, 1999
... SIÇANLARDA, HEMORAJİK ŞOK VE REPERFÜZYON SONRASI GÖRÜLEN ORGAN HASARINDA SİKLOSPORİN A&am... more ... SIÇANLARDA, HEMORAJİK ŞOK VE REPERFÜZYON SONRASI GÖRÜLEN ORGAN HASARINDA SİKLOSPORİN A&amp;amp;#x27;NIN ROLÜ M Süphan Ertürk 1 , Ertuğrul Gazioğlu 1 , Dildar Konukoğlu 1 , Şennur İlvan 1 , Ali Şahin 1 , Osman Şenel 1 , Serap Arıkan 1 , Oğuz Çetinkale ...
BJU International, 2001
Objective To determine variations in the amount of glycosaminoglycans (GAGs) excreted by patients... more Objective To determine variations in the amount of glycosaminoglycans (GAGs) excreted by patients with nocturnal enuresis and/or diurnal incontinence. Patients, subjects and methods The study included 27 patients (aged 5–15 years) with nocturnal enuresis and/or diurnal incontinence, and 27 healthy age‐matched children. Their urinary GAG excretion was assessed over 24 h using the sodium tetraborate‐carbazole method. Results Patients with nocturnal enuresis and/or diurnal incontinence had higher mean values of urinary GAG excretion than age‐matched controls. There were significant differences in GAG excretion between those with nocturnal enuresis and diurnal incontinence and those with nocturnal enuresis alone. Conclusions GAG excretion in patients with nocturnal enuresis and/or diurnal incontinence was significantly higher than in normal children, suggesting that measuring urinary GAGs may be useful in evaluating physiopathological conditions of the bladder wall, and hence in monitor...
Archives of Disease in Childhood, 1999
Archives of Disease in Childhood, 1996
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Papers by Dildar Konukoğlu