File:41467 2023 39758 Fig3.webp

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Endosymbiosis between ''Ixodes ricinus'' and ''Ca.'' Midichloria mitochondrii: FIB-SEM generated 3D datasets of tick oocytes

Summary

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Description
English: Endosymbiosis between Ixodes ricinus and Ca. Midichloria mitochondrii: FIB-SEM generated 3D datasets of tick oocytes.
The individual FIB-SEM micrographs of the wild-type (a–d) and aposymbiotic (e, f) oocytes named based on the vitellogenic stages showing the bacteria (B) and mitochondria (M). g The mitochondria of previtellogenic oocyte were in deformed globular shapes. As vitellogenisation began, mitochondria were globular (h), and remained globular as the vitellin granules accumulated (i, j). In all vitellogenic stages, intramitochondrial and cytosolic bacteria are visible (g–j). The aposymbiotic oocytes (e, f) had tubular mitochondria (k, l), forming an extensive network (w, x), without any bacteria (zc, zd). The 3D reconstructed images of equal oocytes volumes of ~50 μm3 show the distribution of the mitochondrial (yellow (s–x)) and bacterial (green (y–zd)) populations in the cytoplasm (purple) (m–r).
Scale bars: 2 μm n = 4 wild-type and n = 2 aposymbiotic ticks.
Note: aposymbiotic = free of (endo)symbionts
Date
Source Three-dimensional images reveal the impact of the endosymbiont Midichloria mitochondrii on the host mitochondria. In: Nature Communications, volume 14, No. 4133; doi:10.1038/s41467-023-39758-x
Author Zerrin Uzum, Dmitry Ershov, Michael J. Pavia, Adeline Mallet, Olivier Gorgette, Olivier Plantard, Davide Sassera, Fabrizia Stavru
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current09:25, 13 September 2024Thumbnail for version as of 09:25, 13 September 20241,500 × 1,525 (326 KB)Ernsts (talk | contribs)Uploaded a work by Zerrin Uzum, Dmitry Ershov, Michael J. Pavia, Adeline Mallet, Olivier Gorgette, Olivier Plantard, Davide Sassera, Fabrizia Stavru from ''Three-dimensional images reveal the impact of the endosymbiont ''Midichloria mitochondrii'' on the host mitochondria.'' In: ''Nature Communications'', volume 14, No. 4133; doi:10.1038/s41467-023-39758-x with UploadWizard

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