Papers by Sherif M S Hassan
Scottish Medical Journal
Background A recent trend in medical education is developing a more dynamic and integrated curric... more Background A recent trend in medical education is developing a more dynamic and integrated curriculum. Team-based learning (TBL) increases students’ engagement and the active construction of anatomical knowledge. This initial study aimed to empirically observe medical students’ perceptions of their achievement of learning outcomes and the construction of their neuroanatomy knowledge, critical thinking, and problem-solving using an interactive whiteboard (IWB) as a teaching strategy. Methods An independent neuroanatomy lab survey collected students’ perceptions and comments about their learning experiences using the IWB on a questionnaire using a 5-point Likert scale. Results Student participants felt that using the IWB has facilitated their learning experience. 94.2% of student participants endorsed feelings that new technology has helped them achieve their learning outcomes, helped them integrate both their basic science and clinical science/skills knowledge (90.4%), enhanced their...
The FASEB Journal, Oct 3, 2018
Background: Cyclophosphamide (CP) is a chemotherapeutic agent used for treating many neoplastic d... more Background: Cyclophosphamide (CP) is a chemotherapeutic agent used for treating many neoplastic disease. Its application is limited now-a-days due to its multiple side effects including hemorrhagic cystitis. Thymoquinone (TQ) is one of the active ingredients of Nigella sativa seeds, known for its healing potentials. Aim of study: The current study has been carried out to examine the protective effect of 2 different doses of TQ against CP-induced oxidative injury in the urinary bladder in rat. In addition to the possible mechanism through which TQ produces its protective effect. Methods: 5 groups of 40 rats were used in this study. An untreated control group receiving no treatment, a sham control group where TQ (10 mg/kg/day) was administered using gastric gavage, in a single oral dose for 10 consecutive days. A toxicity group where CP-induced toxicity was induced via 100 mg/kg, intraperitoneal for 2 days. In addition to 2 protection groups, both receiving CP at a dose of 100 mg/kg, intraperitoneal for 2 days but receiving 2 different doses of TQ, at concentrations of 10 mg/kg/day for 10 days and 100 mg/kg/day for 5 days. Results: CP caused alteration of the urinary bladder histology in rat, with significant oxidative stress (ROS), as revealed by the reduction in the catalase (CA) and glutathione reductase (GR) in blood, in addition to increased lipid peroxidation to compensate for the ROS as revealed by the increase in the malondialdehyde levels (MDA) in blood. CP also induced cell death, as revealed by the activation of caspase-3 in blood. Post treatment with TQ (at a dose of 100mg/kg/day for 5 consecutive days), however reduced CP-induced ROS and apoptosis. Conclusion: Concomitant use of TQ with CP could ameliorate the CP-induced toxicity on the rat urinary bladder via its antioxidant and antiapoptotic effects.
Cureus
Introduction: Sheet plastination has provided evidence that the posterior atlantooccipital membra... more Introduction: Sheet plastination has provided evidence that the posterior atlantooccipital membrane attaches to the dura's posterior sleeve at the cerebrospinal junction. These findings contradict the traditional anatomical description of this membrane extending from the atlas' posterior arch to the foramen magnum. Methods: A total of 16 plastinated cadavers were studied to evaluate the in situ and gross configuration of the posterior atlantooccipital membrane. Fifteen cadavers underwent sheet plastination, and one head was hemisected and plastinated. In all specimens, stereomicroscopy was used to evaluate the posterior atlantooccipital membrane and related structures within the intervertebral and epidural spaces. Results: In all 16 specimens, the posterior atlantooccipital membrane extending from the occiput, merged with the craniocervical dura mater, and formed a membrane-dura complex that ended at the level of the third cervical vertebra. The superior and inferior myodural bridge coalesced with their respective vertebrodural ligaments and fused with the posterior atlantooccipital membrane at their respective interspaces. Conclusion: The median aspect of the posterior atlantooccipital membrane does not directly communicate with the posterior arch of the atlas. Instead, the posterior atlantooccipital membrane converges with the craniocervical dura mater and terminates at the level of the third cervical vertebra. This membrane-dura complex serves as a common attachment site for the myodural and vertebrodural structures.
Neurourology and urodynamics, Jan 30, 2016
Although once a common antibiotic, polymyxin E fell out of favor after reports of its nephrotoxic... more Although once a common antibiotic, polymyxin E fell out of favor after reports of its nephrotoxicity. However, recent concerns with gram-negative bacteria, which are resistant to multiple antibiotics, have resulted in increased interest in polymyxin E. Silybin is a known antihepatotoxic drug and may have potential for protecting the kidney from polymyxin E. Therefore, the aim of the current study was to evaluate whether silybin affected the damages produced by polymyxin E on the rat kidney. Four groups of rats with 10 rats per group were included in the study: control (no treatment, group I), vehicle (control vehicle treatment, group II), polymyxin E treatment (group III), and polymyxin E and silybin treatment (group IV). Groups II-IV received intravenous treatment twice a day for 7 days. All rats were euthanized after 7 days. Histological, ultrastructural, and morphometric analyses were performed on the rats' kidney tissues. Analysis of tissues from group III showed differences...
The FASEB Journal, Apr 1, 2020
The FASEB Journal, 2016
ObjectivePrevious reports have shown that vitamin K2 could prevent oxidative stress-induced cell ... more ObjectivePrevious reports have shown that vitamin K2 could prevent oxidative stress-induced cell death in neuronal and glial cells in vitro (Sakaue et al., 2011; Li et al., 2003). In addition, it h...
ObjectiveNeuroscience has enjoyed tremendous growth over the past 20 years, including a substanti... more ObjectiveNeuroscience has enjoyed tremendous growth over the past 20 years, including a substantial increase in the number of neuroscience departments, programs, and courses at the undergraduate level. To meet the need of new neuroscience courses, there has also been growth in the number of introductory neuroscience textbooks designed for undergraduates. However, textbooks typically trail current knowledge by five to ten years, especially in neuroscience where our understanding is increasing rapidly. Consequently, it is often important to supplement neuroscience and textbooks with information about recent findings in neuroscience. The purpose of this educational study is to compare 3 different models of teaching and evaluate their effectiveness as they are applied to get a better idea of how neuroscience education is being delivered. Methods3 Models of teaching were compared in this study; The Longitudinal model where the neuroscience course was taught separately, irrespective of th...
Canadian Journal of Physiology and Pharmacology
Acute renal failure complicates renal ischemia–reperfusion (I/R) owing to reactive oxygen species... more Acute renal failure complicates renal ischemia–reperfusion (I/R) owing to reactive oxygen species production. Atorvastatin (ATO) has anti-inflammatory and antioxidant properties. The current study investigated whether ATO alleviated damage induced by renal I/R injury in nondiabetic versus diabetic rat models. Thirty-six rats were equally divided into 6 groups: group A1 (nondiabetic sham), group A2 (nondiabetic I/R), group A3 (nondiabetic ATO + I/R), group B1 (diabetic sham), group B2 (diabetic I/R), and group B3 (diabetic ATO + I/R). All groups experienced 45 min of bilateral renal ischemia followed by 24 h of reperfusion. Groups A3 and B3 were treated with single intraperitoneal doses of ATO (10 mg/kg) 30 min before ischemia. Histological analysis of kidney tissues, kidney function tests, and analyses of caspase-3 and CD44 expression and oxidative stress markers were performed to assess tubular injury. Histological analysis revealed marked tubular damage in groups A2 and B2 but imp...
Neurourology and urodynamics, Jan 3, 2017
Polymyxin E was used for treating gram-negative bacterial infections but not recently for fear of... more Polymyxin E was used for treating gram-negative bacterial infections but not recently for fear of its nephrotoxicity. Silybin has potential to counteract nephrotoxicity; however, few studies have investigated its protective effect on the kidney in an animal model. The purpose of the present study was to assess whether silybin could decrease elevated urine and serum renal biochemical markers induced by polymyxin E in rat kidney. Forty rats were divided randomly into four groups of 10 rats: control (I), vehicle (II), treatment (III, using polymyxin E), and protection (IV, using silybin and polymyxin E). Urine was collected daily for 7 days to test for N-acetyl-beta-D-glucosaminidase (NAG). Serum was collected after euthanizing the rats on day 7 to test kidney functions. RESULTS Group III had significant increases in NAG (all P < 0.001) compared with the other groups, but no differences were found between the other groups. Significant differences in kidney functions were found betwe...
Anatomy & Physiology, 2016
Archives of Medical Science
IntroductionCyclophosphamide (CP) is a chemotherapeutic agent used to treat neoplastic diseases, ... more IntroductionCyclophosphamide (CP) is a chemotherapeutic agent used to treat neoplastic diseases, but its side effects include hemorrhagic cystitis. Thymoquinone (TQ) is an active ingredient of Nigella sativa with healing potential. This study examined the protective effect of TQ against CP-induced oxidative injury in rat urinary bladder.Material and methodsSixty rats were divided into 6 equal groups: untreated control (group A), TQ-treated with 10 mg/kg/day TQ for 10 days (group B1), TQ-treated with 100 mg/kg/day TQ for 5 days (group B2), toxicity control where 100 mg/kg CP was administered for 2 days (group C), CP + TQ-treated with the same TQ treatment as group B1 and 100 mg/kg CP for 2 days (group D1), and CP + TQ-treated with the same TQ treatment as group B2 and 100 mg/kg CP for 2 days (group D2). Rat urinary bladders were assessed histopathologically via hematoxylin and eosin (H&E) and Masson’s trichrome stains and were evaluated for oxidative stress and cell death markers.Res...
Acute restraint stress (ARS) is an unavoidable stress situation and may be encountered in differe... more Acute restraint stress (ARS) is an unavoidable stress situation and may be encountered in different clinical situations. The aim of the current study was to investigate the effects of ARS on the hippocampus and cerebellum, assess the impact of these effects on the behavior and cognitive function, and determine whether pretreatment with ceftriaxone would attenuate the damages produced by ARS on the hippocampus and cerebellum. Four groups of male mice were included in this study: The control group, ARS group, ceftriaxone group, and ARS + ceftriaxone group. Tail suspension test, Y-maze task, and open field tests were used to assess depression, working spatial memory, and anxiety. The biochemical analyses included measurements of serum cortisol, tumor necrotic factor (TNF), interleukin-6, hippocampal expression of bone morphogenetic protein 9 (BMP9), lysosomal-associated membrane protein 1 (LAMP1), glutamate transporter 1 (GLT1), heat shock protein 90, cerebellar expression of S100 protein, glutamic acid decarboxylase (GAD), and carbon anhydrase. Histopathological examination of the brain sections was conducted on the hippocampus and cerebellum by hematoxylin and eosin stains in addition to ultrastructure evaluation using electron microscopy. Our results suggested that ceftriaxone had neuroprotective properties by attenuating the effects of ARS on the hippocampus and cerebellum in mice. This effect was demonstrated by the improvement in the cognitive and behavioral tests as well as by the preservation of the hippocampal and cerebellar architecture.
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Papers by Sherif M S Hassan