Papers by R. Haryo Bimo Setiarto
Journal of ethnic foods, Jul 8, 2024
Journal of ethnic foods, Jul 1, 2024
IOP conference series. Earth and environmental science, Apr 1, 2024
Jurnal Ilmu Pertanian Indonesia, Jan 8, 2024
Sains Malaysiana, Nov 29, 2023
This study explores the probiotic potential of lactic acid bacteria (LAB) in horse milk from Tamb... more This study explores the probiotic potential of lactic acid bacteria (LAB) in horse milk from Tambora, West Nusa Tenggara by examining its functional and probiotic attributes, including bile salt tolerance, low pH, simulated gastric juice (SGJ), simulated intestinal juice (SIJ), antibacterial activity, as well as bile salt hydrolase (BSH) genes. Genotyping of LAB was performed using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) analysis and then identified based on the 16S rRNA gene. A total of 25 LAB isolates showed the ability to grow at low pH, tolerant to bile, and survived under SIJ and SGJ conditions. The BSH gene was confirmed in three isolates, namely: SK1-28, SK2-30, and SK2-34. Results of RFLP-PCR analysis showed that the LAB isolates were grouped into three groups based on the number and molecular weight of the differences DNA fragments. The 16S rRNA analysis showed that the first two groups were Lacticaseibacillus rhamnosus, whereas the third group was Lactiplantibacillus plantarum. In addition, all Lacticaseibacillus rhamnosus isolates in group I showed the ability to grow at pH 9.0, but not group II. It can be concluded that Lacticaseibacillus rhamnosus and Lactiplantibacillus plantarum can be used as the indigenous probiotic bacteria source from Indonesia.
AIP Conference Proceedings, Dec 31, 2022
IOP Conference Series: Earth and Environmental Science, Nov 30, 2023
Journal of Ethnic Foods, Nov 30, 2023
Jurnal Penelitian Pendidikan IPA (JPPIPA), Nov 24, 2023
Current Applied Science and Technology, Aug 28, 2023
E3S web of conferences, Dec 31, 2022
Wikrama Parahita : Jurnal Pengabdian Masyarakat, May 17, 2024
Biodiversitas, May 5, 2023
Various human activities emit carbon dioxide which drives global warming and climate change. Seag... more Various human activities emit carbon dioxide which drives global warming and climate change. Seagrass ecosystems can absorb and store enormous quantities of carbon, hence reducing carbon emissions. This research aimed to assess the diversity, vegetation cover, carbon stock and associated biota of seagrass beds in Central Tapanuli District, North Sumatra, Indonesia. Purposive sampling method was employed with data collection used the line transect quadrant method. Carbon stock was measured using the loss of ignition method. There were four species of seagrass in the studied area, namely Enhalus acoroides (L.f.) Royle, Thalassia hemprichii (Ehrenb. ex Solms) Asch., Cymodocea rotundata Asch. & Schweinf. and Halodule uninervis (Forssk.) Boiss. with a density ranging from 23-164 stands/m 2. Seagrass cover ranged from 3.87%-34.37%. Associated biota (fish and crustaceans) at each observation station were Siganus
Journal of Ethnic Foods
Indonesia, a country with diverse cultures, possesses a rich variety of cultural heritage. The ai... more Indonesia, a country with diverse cultures, possesses a rich variety of cultural heritage. The aim of this review is to discuss the culture of lepet considering its ethnic perspective, preparation and consumption, nutritional and functional properties, safety, and future outlook aspects. Lepet, a traditional Javanese dish, consists of sticky rice, grated young coconut, coconut milk, cowpea, and salt. It is wrapped in young coconut leaves with a specific pattern, boiled, soaked, and then hung to cool and dry. The persuasive technique employed by Sunan Kalijaga, which combines Javanese cultural traditions and Islamic religious teachings, symbolizes lepet for the Javanese people. This method facilitated the spread of Islam by emphasizing the importance of purity and refraining from committing further mistakes after apologizing during the Eid Al-Fitri tradition. Lepet is rich in carbohydrates and some protein. It is gluten-free and offers benefits for bone and skin health due to its con...
Philippine Journal of Science
Debranching is one of the enzymatic modifications of starch widely used in analyzing the increase... more Debranching is one of the enzymatic modifications of starch widely used in analyzing the increased levels of resistant starch (RS) in foodstuffs. However, this modification has a varying effect on each type of high-carbohydrate food. This study aims to analyze the effect of the debranching pullulanase modification in increasing the levels of RS and prebiotic properties of high-carbohydrate foods. This study used 28 articles that were analyzed and selected through the PRISMA guide method from 10.302 selected libraries. Secondary data will be analyzed based on the percentage of Effect Size Hedges’d (standardized mean difference/ SMD) and confidence interval (CI) values using OpenMEE software. The results showed that the pullulanase debranching enzyme method significantly increased levels of RS and prebiotic properties (SMD 2.395; CI 95% p < 0.001). In conclusion, this study confirmed that the pullulanase debranching enzyme method had a significant effect with a 95% CI in increasing...
Tropical Life Sciences Research
Probiotics are a non-digestible food ingredient that promotes the growth of beneficial microorgan... more Probiotics are a non-digestible food ingredient that promotes the growth of beneficial microorganisms in the intestines. One of the functional food ingredients, Momala corn flour, is a source of prebiotics with a resistant starch content of 4.42%. This study aimed to improve the prebiotic properties and resistant starch content of modified corn flour (MCF) Momala Gorontalo by using physical, chemical, and enzymatic modification processes. The research methods include physical modification (heat moisture treatment, annealing, autoclaving-cooling cycling, microwave), chemical modification (acid hydrolysis), and enzymatic modification (debranching pullulanase). The results showed that the modified by heat moisture treatment (HMT) increased RS levels 1-fold, annealing modification (ANN) 8.9-fold, autoclaving-cooling one cycle modification (AC-1C) 2.9-fold, autoclaving-cooling two cycles modification (AC-2C) 2.0-fold, microwave modification (MW) 1.3-fold, acid hydrolysis (HA) modificatio...
Reviews in Agricultural Science
Probiotics are beneficial live microorganisms because they provide health benefits to the host af... more Probiotics are beneficial live microorganisms because they provide health benefits to the host after being consumed in sufficient amounts, primarily by increasing the proliferation of native digestive microflora. Hypercholesterolemia is a condition in which cholesterol serum concentration is elevated significantly (exceeds 200 mg/dL). Hypercholesterolemia is characterized by high plasma levels of low-density lipoprotein (LDL) and low plasma concentration of high-density lipoprotein (HDL). This review aims to examine the mechanism of action of probiotics in preventing the risk of hypercholesterolemia and reducing LDL cholesterol levels in the human body. The mechanism of cholesterol-lowering effect of probiotic bacteria can be classified into four basic principles such as 1) the conversion of cholesterol to coprostanol, 2) the deconjugation of cholesterol by probiotics' bile salt hydrolase enzyme, 3) the alteration of protein expression related to cholesterol synthesis, and 4) the production of Short Chain Fatty Acids (SCFA) by probiotics. Cholesterol converted to coprostanol is not absorbed by the small intestine and is directly excreted with the feces. The deconjugated cholesterol is easily precipitated and thus not absorbed by the small intestine. It also induces the assimilation of cholesterol into the probiotic membrane. Finally, the SCFAs, produced by probiotics fermentation within host digestive tract, can upregulate the synthesis of HDL apolipoprotein.
African Journal of Food, Agriculture, Nutrition and Development
Lore Lindu National Park was the most important flora and fauna protected area in Central Sulawes... more Lore Lindu National Park was the most important flora and fauna protected area in Central Sulawesi, Indonesia. This area has high biodiversity, one of which is edible macro fungi. Macro fungi have attracted worldwide attention and reputation because of their diverse functions including beauty and aesthetics, medicinal effects (anticancer, antidiabetic, immunoenhancing and antioxidant), cosmetic ingredients, high nutritional value as food, economic value and ecosystem services. Macro fungi were rich in essential minerals, micro elements, vitamins, protein, carbohydrates and fiber. Indigenous peoples around this area have long been using edible macro fungi that grow wild in the forest both as a source of food and medicine. This study aimed to analyze the effect of cooking on the proximate composition and mineral content of several edible macro fungi originating from the Lore Lindu National Park area (Auricularia sp, Auricularia auricula-judae, Termitomyces sp, Lentinus sp, Pleurotus o...
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Papers by R. Haryo Bimo Setiarto