Papers by hamid piroozmanesh
Revista Internacional de Andrología
Anatomy & Cell Biology
We aimed to investigating the effects of metformin (MET) in combination with alpha lipoic acid (A... more We aimed to investigating the effects of metformin (MET) in combination with alpha lipoic acid (ALA) on hormonal and biochemical parameters, in polycystic ovary syndrome (PCOS) women undergoing intracytoplasmic sperm injection (ICSI). This experimental pilot study with a randomized design was carried out on 40 PCOS women in two groups: (1) MET group, administered 1,500 mg/day MET, and (2) MET (1,500 mg/day)+ALA (1,800 mg/day) group. Drugs were administered from the third day of the previous cycle until the day of oocyte aspiration (six weeks of treatment in total). MET+ALA significantly increased the number of maturated oocytes and the rate of fertilization when compared to the MET group. Combination MET+ALA could increase significantly the number of oocytes retrieval and the number of good-quality embryos. Also, the malondialdehyde (MDA) level decreased significantly in the MET+ALA group and the total antioxidant capacity (TAC) level increased significantly in the MET+ALA group compared to the MET group. Also, fasting blood sugar (FBS), insulin, luteinizing hormone (LH), and LH/follicle stimulating hormone (FSH) levels were significantly lower in the MET+ALA group. The pregnancy outcomes showed no significant difference in the rates of biochemical pregnancy, clinical pregnancy, miscarriage, and live births between the control and study groups. The combination of MET+ALA treatment could moderate the complications of PCOS and subsequently improve oocyte and embryo quality.
Research in Molecular Medicine
Background: This study aimed to explore whether the addition of a cyclic adenosine monophosphate ... more Background: This study aimed to explore whether the addition of a cyclic adenosine monophosphate (cAMP) analog and isobutylmethylxanthine (IBMX) in freezing media improved sperm quality and what role cAMP has in this recovery. Materials and methods: ach semen sample was cryopreserved into four groups: fresh semen sample, as a control group, freezing medium + 2.5 mM cAMP analog and 0.2 mM IBMX, freezing medium + 12.5 mM cAMP analog and 0.2 mM IBMX, and freezing medium + 25 mM cAMP analog and 0.2 mM IBMX. Sperm parameters after post-thaw were analyzed according to WHO instruction (2010). Viability, acrosome reaction, and DNA damage levels of the samples were evaluated. Results: Our results indicated that the effective concentrations of 12.5 and 25 mM cAMP analog and 0.2 mM IBMX significantly improved the total motility, progressive motility, and viability of the frozen-thawed (P<0.05). However, non-progressive motility and immotile were significantly reduced in the 12.5 and 25 mM c...
Pharmaceutical and Biomedical Research
Background: Arsenic is a heavy metal that causes malformation. It affects the reproductive system... more Background: Arsenic is a heavy metal that causes malformation. It affects the reproductive system of men by induction of oxidative stress. Objectives: This study investigated the effect of Vitamin C (Vit C), as a potent antioxidant, on sperm quality and testicular tissue in mice that received sodium arsenite. Methods: A total of 28 adult male NMRI mice were divided into four groups: control, sodium arsenite (7 mg/kg/d), Vitamin C (Vit C; 150 mg/kg/d), and sodium arsenite+Vit C (with previous doses). Gavage treatments were administered for 35 days. At the end of the treatments, the right testis was used for histopathological observations and apoptosis assessment, and the left testis to evaluate sperm parameters. Results: Sperm parameters such as count, motility, viability, morphology, tail length, Daily Sperm Production (DSP), the diameter of seminiferous tubules, the height of the germinal epithelium, and the number of germ cells and Sertoli cells were significantly decreased in the...
Jentashapir Journal of Cellular and Molecular Biology, 2021
Background: Coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory s... more Background: Coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may lead to the infertility of men. Objectives: This study aimed to investigate the impact of COVID-19 infection on sperm parameters and reproductive hormones in fertile men. Methods: A total of 100 males were selected and divided into two groups: (1) patients in convalescence (patients suffering from COVID-19 infection in pharyngeal swab in accordance with reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); (2) negative control group (without antibodies). Semen and blood samples were gathered from all subjects. In the native semen, immunoglobulin (Ig) M and IgG antibodies in the blood were confirmed, and COVID-19 was detected via RT-PCR. To this end, based on the World Health Organization (WHO) guidelines, semen analysis, total antioxidant capacity (TAC), and sperm DNA integrity were assessed. Results: Results demonstrated that spe...
Andrologia, 2021
In infertile men, reduced expression of heat shock protein A2 (HSPA2) is related to reduced sperm... more In infertile men, reduced expression of heat shock protein A2 (HSPA2) is related to reduced sperm quality and function. The present study has aimed to investigate the effects of N‐acetyl‐cysteine (NAC) supplementation on expression of heat shock protein A2 (HSPA2). In this study in continuation of previous study, semen samples from 50 astheno‐terato‐zoospermic men who have received NAC (600 mg/day) orally for three months were evaluated for expression HSPA2 using RT‐PCR, and Western blot analysis. In addition, semen samples of these individuals were assessed for sperm parameters, DNA fragmentation (TUNEL), protamine deficiency (CMA3), lipid peroxidation index (MDA) and total antioxidant capacity (TCA). All assessment was carried out before and after NAC treatment. In addition to improved sperm parameters and aforementioned functional parameters, the presented results revealed the significant increase in relative expression levels of HSPA2 was obtained after using NAC treatment (p < .05). Correlation analysis also demonstrated that HSPA2 expression is significantly related to most of the assessed parameters. NAC may directly or indecently impose its beneficial effect through increased expression of HSPA2, which plays a potential role in proper folding of element needed to counteract stress condition in infertile individuals.
Background & Aims: Varicocele is among the most common identifiable abnormality found in men eval... more Background & Aims: Varicocele is among the most common identifiable abnormality found in men evaluated for infertility. Despite the long history associated with varicoceles, there remains much controversy regarding their diagnosis and management. The aim of this study was to determine association between varicocele, sperm parameters, and embryo quality of ICSI outcome in infertile men. Materials & Methods: Fifty individuals with varicocele for Intracytoplasmic sperm injection (ICSI) treatment were included in this study. The control group included healthy men without varicoceles (n=50). Semen samples were analyzed according to the World Health Organization (WHO) criteria. Hormonal analysis of serum LH, FSH, and testosterone were measured. Sperm DNA fragmentation was assessed by SCD (Halo sperm), and then ICSI fertilization rate, percentage of highquality embryos and chemical pregnancy rate were measured. Results: In semen analyses, the total sperm count, total motile sperm and norma...
Qom Univ Med Sci J, 2021
Background and Objectives: The semen cryopreservation is not only required for improving the succ... more Background and Objectives: The semen cryopreservation is not only required for improving the success chance of assisted reproductive technologies fertility but also is performed for men undergoing chemotherapy, radiotherapy, and surgery, and facing ejaculation problems. This study aimed to investigate the effects of N-acetylcysteine on human sperm parameters and DNA damage in frozenthawed sperm samples of patients with asthenozoospermia. Methods: In this study, 20 patients with asthenozoospermia referring to Jihad University Infertility Treatment Center, Qom, Iran, were evaluated in three groups, namely control, freezing, and freezing + Nacetyl-cysteine (1mg/ml). Sperm parameters, viability, and DNA damage were respectively assessed using the World Health Organization guideline (2010), eosin-nigrosin staining, and sperm chromatin dispersion kit, respectively in all three groups. The statistical analysis was performed using one-way ANOVA and Tukey post hoc test. The significance level was considered at the p-value of < 0.05. Results: The freezing process resulted in a decrease in sperm motility parameters, and the addition of the antioxidant N-acetyl-cysteine improved sperm motility, morphology, and sperm viability. The addition of N-acetyl-cysteine could reduce DNA damage (P<0.05). Conclusion: Our results showed that N-acetyl-cysteine could reduce the disruptive effects of the freezing-thawing process.
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Papers by hamid piroozmanesh