Papers by Spiros Drivelos
Determination of geographical origin of food and beverages has been a growing issue over the past... more Determination of geographical origin of food and beverages has been a growing issue over the past decade for all countries around the world, mostly because of the concern of consumers about the authenticity of the food that they eat. An increasing number of research articles in the past five years have investigated the elemental composition and the isotope ratios as indicators to determine the origin of food and beverages. The indicators commonly used are stable-isotope ratios of hydrogen, oxygen, nitrogen, carbon and sulfur, combined with isotope ratios of strontium and lead, and elemental concentrations.
Analytical and bioanalytical …, Jan 1, 2010
A new hydrophilic interaction liquid chromatographic (HILIC) method for the simultaneous determin... more A new hydrophilic interaction liquid chromatographic (HILIC) method for the simultaneous determination of isoascorbic (IAA) and ascorbic acid (AA) was developed. The separation of IAA and AA was studied in various HILIC stationary phases and the influence of the composition of the mobile phase, the ionic strength and the column temperature to the chromatographic characteristics is presented. The final method used an aminopropyl column under isocratic elution with acetonitrile-100 mM ammonium acetate solution (90:10, v/v) at a flow rate of 0.4 mL/min and a detection wavelength of 240 nm. This method was validated and the calibration curves were found to be linear in the range of 1.0-65 μg/mL for both IAA and AA. The method limit of detection for IAA determination in fish tissue was 2.3 μg/g. Inter-day precision (as %RSD R ) was ranged between 0.56% and 8.3% at three concentration levels, whereas the respected recoveries ranged between 82% and 98%. This method was applied to the determination of IAA (as additive E315) in frozen redfish samples. The hyphenation of the HILIC separation with a tandem mass spectrometer was also studied and the problems encountered with negative electrospray ionization under HILIC separation conditions are discussed.
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Papers by Spiros Drivelos